Publication detail

Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles

KOŠELOVÁ, Z. FOHLEROVÁ, Z.

Original Title

Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles

Type

conference paper

Language

English

Original Abstract

This research delves into the realm of biosensor improvement through the utilization of gold nanoparticles (Au NPs). The primary objective is to assess the impact of different-sized Au NPs on sensor performance, specifically investigating whether 100 nm or 20 nm nanoparticles prove more favourable to enhancement. Moreover, we aim to inspect the biosensor's response to varied concentrations of Au NPs, unravelling the involved collaboration between nanoparticle size, concentration, and overall sensor properties. This modification of commercial electrodes with Au NPs, could be way for enhancing surface area and enzyme immobilization. Notably, the investigation also explores the potential drawbacks associated with increasing nanoparticle concentration and offers insights into optimizing biosensor design. It has been observed that while 20 nm Au NPs slightly decreased impedance values at higher glucose concentrations, 100 nm Au NPs, conversely, exhibited an increase in capacitive behaviour. Equally crucial is the parameter chosen for constructing the calibration curve. From impedance values at low frequencies of alternating voltage, such as 2 Hz, a lower Limit of Detection (LOD) is obtained. However, the analysis of Rct in the case of 20 nm Au NPs reveals a broader range of glucose concentrations falling within the calibration area. Through a comprehensive analysis of electrochemical behaviour, impedance, and charge transfer resistance, we endeavour to provide contributions to the improvement of biosensor technologies.

Keywords

gold nanoparticles, biosensors, enzyme immobilization, impedance spectroscopy, glucose detection, calibration curves

Authors

KOŠELOVÁ, Z.; FOHLEROVÁ, Z.

Released

23. 4. 2024

Publisher

Brno University of Technology, Faculty of Electrical Engineering

Location

Brno

ISBN

978-80-214-6230-4

Book

Proceedings II of the 30 th Conference STUDENT EEICT 2024

Edition

1

ISBN

2788-1334

Periodical

Proceedings II of the Conference STUDENT EEICT

Year of study

30

Number

1

State

Czech Republic

Pages from

202

Pages to

206

Pages count

5

URL

https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_2.pdf

BibTex

@inproceedings{BUT189256,
  author="Zuzana {Košelová} and Zdenka {Fohlerová}",
  title="Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles",
  booktitle="Proceedings II of the 30 th Conference STUDENT EEICT 2024",
  year="2024",
  series="1",
  journal="Proceedings II of the Conference STUDENT EEICT",
  volume="30",
  number="1",
  pages="202--206",
  publisher="Brno University of Technology, Faculty of Electrical Engineering",
  address="Brno",
  isbn="978-80-214-6230-4",
  issn="2788-1334",
  url="https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_2.pdf"
}