Publication detail

Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages

Pavlína Šimečková, František Hubatka, Jan Kotouček, Pavlína Turánek Knötigová, Josef Mašek, Josef Slavík, Ondrej Kováč, Jiří Neča, Pavel Kulich, Dominik Hrebík, Jana Stráská, Kateřina Pěnčíková, Jiřina Procházková, Pavel Diviš, Stuart Macaulay, Robert Mikulík, Milan Raška, Miroslav Machala & Jaroslav Turánek

Original Title

Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages

Type

journal article in Web of Science

Language

English

Original Abstract

Gadolinium (Gd)–based contrast agents are extensively used for magnetic resonance imaging (MRI). Liposomes are potential nanocarrier–based biocompatible platforms for development of new generations of MRI diagnostics. Liposomes with Gd–complexes (Gd–lip) co–encapsulated with thrombolytic agents can serve both for imaging and treatment of various pathological states including stroke. In this study, we evaluated nanosafety of Gd–lip containing PE-DTPA chelating Gd+3 prepared by lipid film hydration method. We detected no cytotoxicity of Gd–lip in human liver cells including cancer HepG2, progenitor (non–differentiated) HepaRG, and differentiated HepaRG cells. Furthermore, no potential side effects of Gd–lip were found using a complex system including general biomarkers of toxicity, such as induction of early response genes, oxidative, heat shock and endoplasmic reticulum stress, DNA damage responses, induction of xenobiotic metabolizing enzymes, and changes in sphingolipid metabolism in differentiated HepaRG. Moreover, Gd–lip did not show pro–inflammatory effects, as assessed in an assay based on activation of inflammasome NLRP3 in a model of human macrophages, and release of eicosanoids from HepaRG cells. In conclusion, this in vitro study indicates potential in vivo safety of Gd–lip with respect to hepatotoxicity and immunopathology caused by inflammation.

Keywords

gadolinium, nanoliposomes

Authors

Pavlína Šimečková, František Hubatka, Jan Kotouček, Pavlína Turánek Knötigová, Josef Mašek, Josef Slavík, Ondrej Kováč, Jiří Neča, Pavel Kulich, Dominik Hrebík, Jana Stráská, Kateřina Pěnčíková, Jiřina Procházková, Pavel Diviš, Stuart Macaulay, Robert Mikulík, Milan Raška, Miroslav Machala & Jaroslav Turánek

Released

16. 3. 2020

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

10

Number

4780

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

13

Pages count

13

URL

https://www.nature.com/articles/s41598-020-60284-z.pdf

BibTex

@article{BUT163205,
  author="Jaroslav {Turánek} and Pavel {Diviš}",
  title="Gadolinium labelled nanoliposomes as the platform for MRI theranostics: in vitro safety study in liver cells and macrophages",
  journal="Scientific Reports",
  year="2020",
  volume="10",
  number="4780",
  pages="1--13",
  doi="10.1038/s41598-020-60284-z",
  issn="2045-2322",
  url="https://www.nature.com/articles/s41598-020-60284-z.pdf"
}