Project detail

Labelling of proteins with quantum dots and their sensing using microfluidic system

Duration: 01.02.2013 — 31.12.2015

Funding resources

Czech Science Foundation - Postdoktorandské granty

- whole funder (2013-02-01 - not assigned)

On the project

Description in English
Using of fluorescent nanoparticles as probes for bioanalytical applications is highly promising, because fluorescence-based techniques are very sensitive. Quantum dots (QDs) seem to show the greatest promise as labels for tagging and imaging in biological systems owing to their impressive photostability, which allow long-term observations of biomolecules. The proposal project is aimed at research of QDs and their application in microfluidic system and capillary electrophoresis for selective detection of proteins. Optimization of preparation conditions of various fluorescent QDs in aqueous solution will be achieved by novel approaches of synthesis in reflux condenser or in microwave reactor. In the next step, the synthesized QDs will be modified with biochemical agents due to their binding with proteins via two strategies, namely using biotin to provide biotinylated QDs which can be employed in specific labelling strategies or using QDs functionalization with antibodies.

Key words in English
quantum dots, biofuncionalization, microfluidic

Mark

GP13-20303P

Default language

Czech

People responsible

Pekárková Jana, Ing., Ph.D. - principal person responsible

Units

Centre of Sensor, Information and Communication Systems
- (2013-02-01 - not assigned)

Results

CHOMOUCKÁ, J.; DRBOHLAVOVÁ, J.; MAJZLÍKOVÁ, P.; PRÁŠEK, J.; PEKÁREK, J.; HRDÝ, R.; HUBÁLEK, J. Conjugation Bovine Serum Albumine with CdTe Quantum Dots. In Conference Proceedings 5th International Conference NANOCON 2013. 1. Ostrava: Tanger, Ltd., 2013. p. 566-571. ISBN: 978-80-87294-47-5.
Detail

CHOMOUCKÁ, J.; DRBOHLAVOVÁ, J.; VACULOVIČOVÁ, M.; MAJZLÍKOVÁ, P.; PRÁŠEK, J.; PEKÁREK, J.; HUBÁLEK, J. Study of Protein Conjugation with Different Types of CdTe Quantum Dots. Conference Proceedings - ICQNM 2013. 1. Barcelona: IARIA, 2013. p. 18-22. ISBN: 978-1-61208-303-2.
Detail

DRBOHLAVOVÁ, J.; PEKÁRKOVÁ, J.; HRDÝ, R.; SVATOŠ, V.; HUBÁLEK, J. Biosensing surfaces based on quantum dots array. In 4th International Conference on Nanotechnology and Biosensors. Applied Mechanics and Materials. Switzerland: Trans Tech Publications Inc., 2014. p. 1602-1606. ISBN: 978-3-03835-001-9. ISSN: 1660-9336.
Detail

MIHAJLOVIĆ, A.; PEKÁRKOVÁ, J.; HUBÁLEK, J. Core/shell quantum dots as fluorescent labels of biomolecules. XV. Workshop of Physical Chemists and electrochemists. Book of abstracts. Brno: Masaryk University, 2015. p. 122-125. ISBN: 978-80-210-7857-4.
Detail

PEKÁRKOVÁ, J.; FOHLEROVÁ, Z.; DRBOHLAVOVÁ, J.; PEKÁREK, J.; MAJZLÍKOVÁ, P.; PRÁŠEK, J.; HUBÁLEK, J. Synthesis of CdTe/ZnZ Core/Shell Quantum Dots and Their In Vitro Toxicity Assessment. In 6 th International conference Nanocon 2014. 1. Ostrava: Tanger, Ltd., 2014. p. 664-669. ISBN: 978-80-87294-53-6.
Detail

ŠOBROVÁ, P.; BLAŽKOVÁ, I.; CHOMOUCKÁ, J.; DRBOHLAVOVÁ, J.; VACULOVIČOVÁ, M.; KOPEL, P.; HUBÁLEK, J.; KIZEK, R.; ADAM, V. Quantum dots and prion proteins Is this a new challenge for neurodegenerative diseases imaging?. PRION, 2013, vol. 7, no. 5, p. 349-358. ISSN: 1933-6896.
Detail

PEJOVIČ SIMEUNOVIČ, J.; PEKÁRKOVÁ, J.; ŽÁK, J.; CHAMRADOVÁ, I.; HUBÁLEK, J. Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein. Monatshefte für Chemie, 2017, vol. 148, no. 11, p. 1901-1909. ISSN: 1434-4475.
Detail

STANISAVLJEVIC, M.; PEKÁRKOVÁ, J.; DOSTÁLOVÁ, S.; KŘÍŽKOVÁ, S.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R. Interactions between CdTe quantum dots and DNA revealed by capillary electrophoresis with laser-induced fluorescence detection. Electrophoresis, 2014, vol. 35, no. 18, p. 2587-2592. ISSN: 0173-0835.
Detail

ŽÁK, J.; SEDLÁČEK, J.; PEJOVIC, J.; PEKÁRKOVÁ, J.: HVElektroforezniZdroj; VN zdroj pro řízení kapilární elektroforézy. Technická 10, 0.66. URL: http://www.umel.feec.vutbr.cz/LabSensNano/products.aspx?id=91. (funkční vzorek)
Detail

ŽÁK, J.; KOSINA, P.; CHOMOUCKÁ, J.; HUBÁLEK, J.: FluorescencniOsvit; Osvitová jednotka pro fluorescenční měření. Technická 10, 0.68. URL: http://www.umel.feec.vutbr.cz/LabSensNano/products.aspx?id=78. (funkční vzorek)
Detail

SEDLÁČEK, J.; ŽÁK, J.; PEKÁREK, J.; CHOMOUCKÁ, J.; HUBÁLEK, J.: FluorescencniElektroforeza; Inovace měřicí komory pro fluorescenční měření. Technická 10, 0.68. URL: http://www.umel.feec.vutbr.cz/LabSensNano/products.aspx?id=77. (funkční vzorek)
Detail

Responsibility: Pekárková Jana, Ing., Ph.D.