Project detail

Laser-induced breakdown spectroscopy as a promising readout method for magnetic microbeads-based immunoassay

Duration: 01.03.2023 — 28.02.2024

Funding resources

Brno University of Technology - Vnitřní projekty VUT

- whole funder (2023-01-01 - 2024-12-31)

On the project

Laser-induced breakdown spectroscopy (LIBS) has shown a big potential in nanoparticle-based immunoassay. Compared to other conventional readout methods it provides fast, multi-elemental analysis and spatial mapping with high lateral resolution. Moreover, labels without fluorescence properties can be used. In this specific research, we will explore LIBS application in magnetic magnetic-based immunoassay format, the alternative immunoassay surface, and multiplexing.

Mark

CEITEC VUT-J-23-8207

Default language

Czech

People responsible

Kaiser Jozef, prof. Ing., Ph.D. - fellow researcher
Kopřivová Hana, Ing. - fellow researcher
Vytisková Karolína, Mgr. - principal person responsible

Units

Advanced instrumentation and methods for material characterization
- beneficiary (2023-03-01 - 2024-02-28)
Central European Institute of Technology BUT
- responsible department (2023-01-18 - 2023-03-06)

Results

KOPŘIVOVÁ, H.; KISS, K.; BRUNNBAUER, L.; KRBAL, L.; STEJSKAL, V.; BUCHTOVÁ, M.; KAŠKA, M.; LIMBECK, A.; POŘÍZKA, P.; KAISER, J. Advanced correlative imaging of malignant melanomas using LIBS, LA-ICP-MS and immunohistochemistry. 2023.
Detail

KOPŘIVOVÁ, H.; KISS, K.; BUDAY, J.; BRUNNBAUER, L.; BUCHTOVÁ, M.; LIMBECK, A.; POŘÍZKA, P.; KAISER, J. Techniky laserové ablace a imunohistochemie pro korelativní analýzu maligních melanomů. 2023.
Detail

KOPŘIVOVÁ, H.; KISS, K.; BRUNNBAUER, L.; KRBAL, L.; STEJSKAL, V.; BUCHTOVÁ, M.; KAŠKA, M.; LIMBECK, A.; POŘÍZKA, P.; KAISER, J. Correlative Imaging of Malignant Melanomas Using Laser-Based Techniques, Histology, and Immunohistochemistry. 2023.
Detail

ZIKMUNDOVÁ, E.; SKLENÁROVÁ, D.; KOČÍ, E.; ZATLOUKALOVÁ, T.; BAČOVÁ, T.; MAKHNEVA, E.; HOLUB, D.; MACHAČOVÁ, E.; KOPŘIVOVÁ, H.; VYTISKOVÁ, K.; POŘÍZKA, P.; NOVOTNÝ, K.; SKLÁDAL, P.; FARKA, Z.; KAISER, J. Magnetic microbead-based upconversion immunoassay with laser-induced breakdown spectroscopy readout for the detection of prostate-specific antigen. MICROCHIMICA ACTA, 2024, vol. 191, no. 11, ISSN: 1436-5073.
Detail

KOPŘIVOVÁ, H.; KISS, K.; KRBAL, L.; STEJSKAL, V.; BUDAY, J.; POŘÍZKA, P.; KAŠKA, M.; RYŠKA, A.; KAISER, J. Imaging the elemental distribution within human malignant melanomas using Laser-Induced Breakdown Spectroscopy. Analytica Chimica Acta, 2024, vol. 1310, no. 6, p. 1-8. ISSN: 1873-4324.
Detail

KISS, K.; KOPŘIVOVÁ, H.; STEJSKAL, V.; KRBAL, L.; BUDAY, J.; BRUNNBAUER, L.; KÉPEŠ, E.; POŘÍZKA, P.; RYŠKA, A.; KAŠKA, M.; KAISER, J.; LIMBECK, A. Assessing spatial distribution of bioindicator elements in various cutaneous tumors using correlative imaging with laser-ablation-based analytical methods. TALANTA, 2024, vol. 279, no. 126651, ISSN: 1873-3573.
Detail

FARKA, Z.; VYTISKOVÁ, K.; MAKHNEVA, E.; ZIKMUNDOVÁ, E.; HOLUB, D.; BUDAY, J.; PROCHAZKA, D.; NOVOTNÝ, K.; SKLÁDAL, P.; POŘÍZKA, P.; KAISER, J. Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles. Analytica Chimica Acta, 2024, vol. 1299, no. 342418, ISSN: 1873-4324.
Detail

Responsibility: Vytisková Karolína, Mgr.