Project detail

Analysis of the minor ampholytes from a biological mixtures by carrier ampholyte free isoelectric focusing

Duration: 01.01.2000 — 31.12.2002

Funding resources

Czech Science Foundation - Standardní projekty

- whole funder (2000-01-01 - 2002-12-31)

On the project

Description in English
The aim of the project is further development of carrier ampholytes free isoelectric focusing as a tool for the analysis of peptides and proteins and another ampholytes from complex biological mixtures, in the pH region far away from neutrality, withoutdenaturation of separated compounds and with possibility of trapping minor components in the low concentration up to 10-9 mole/l. Suggested method enables isoelectric micropreparation of substances in 100 g quantities from the sample, where such a substance is in concentration 1 mg/kg. A part of the project is theoretical description of the method, determination of its limits, development of a necessary equipment, experimental verification of the method on practical samples and comparison of the resultswith similar electrophoretic methods if such a comparison can be done.

Mark

GA203/00/0251

Default language

Czech

People responsible

Bejček Ludvík, doc. Ing., CSc. - principal person responsible

Units

Department of Control and Instrumentation
- (2001-01-01 - not assigned)

Results

ŠTOHL, R. Machine Vision Applicated on the Zone Detection for Isotachophoresis. In Proceedings of 7th Conference STUDENT FEI 2001. Brno: Vysoké učení technické v Brně, FEI, 2001. p. 30 ( p.)ISBN: 80-214-1860-5.
Detail

ŠTOHL, R. Machine Vision Applicated on the Zone Detection of Electroseparation Methods. In Artificial Intelligence in Control and Measurement. Brno: Vysoké učení technické v Brně, FEI, 2000. p. 120 ( p.)ISBN: 80-214-1641-6.
Detail

ŠTOHL, R.; GLOVINOVÁ, E.; POSPÍCHAL, J. Detection system for electro-separation analytical methods. JOURNAL OF SEPARATION SCIENCE, 2005, vol. 28, no. 12, p. 1363 ( p.)ISSN: 1615-9314.
Detail

ŠTOHL, R., ZEZULKA, F. Machine Vision applied on the Zone Detection for Isotachophoresis. In ICCC 2004. Zakopane: University of Science and Technology, Poland, 2004. p. 1 ( p.)
Detail

Radek Štohl, Eliška Glovinová and Jan Pospíchal. Detection System for Electro-separation Analytical Methods. In Proceedings of the International Conference CEEPUS Summer School 2005. Brno: Brno University of Technology, 2005. p. 120 ( p.)ISBN: 80-214-2976-3.
Detail

ŠTOHL, R. Sliding Detection System for Electroseparation Methods. In Proceedings of 9th Conference and Competition Student EEICT 2003. Brno: Ing. Zdeněk Novotný, CSc., 2003. p. 267 ( p.)ISBN: 80-214-2378-1.
Detail

Responsibility: Bejček Ludvík, doc. Ing., CSc.