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KREJČOVÁ, L. HYNEK, D. KOPEL, P. ADAM, V. HUBÁLEK, J. TRNKOVÁ, L. KIZEK, R.
Original Title
Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs
Type
journal article in Web of Science
Language
English
Original Abstract
In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine–adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 degrees Celsius, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 ug mL 1. The detection limit (3 signal/noise) was estimated as 15 ng ml 1 of ODN-SH-Cd.
Keywords
paramagnetic particles, DNA, differential pulse voltammetry, square wave voltammetry
Authors
KREJČOVÁ, L.; HYNEK, D.; KOPEL, P.; ADAM, V.; HUBÁLEK, J.; TRNKOVÁ, L.; KIZEK, R.
RIV year
2013
Released
1. 4. 2013
ISBN
0009-5893
Periodical
CHROMATOGRAPHIA
Year of study
76
Number
7-8
State
Federal Republic of Germany
Pages from
355
Pages to
362
Pages count
8
BibTex
@article{BUT100954, author="Ludmila {Krejčová} and David {Hynek} and Pavel {Kopel} and Vojtěch {Adam} and Jaromír {Hubálek} and Libuše {Trnková} and René {Kizek}", title="Paramagnetic particles isolation of influenza oligonucleotide labelled with CdS QDs", journal="CHROMATOGRAPHIA", year="2013", volume="76", number="7-8", pages="355--362", doi="10.1007/s10337-012-2327-0", issn="0009-5893" }