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VYSLOUŽILOVÁ, L. KŘÍŽKOVÁ, S. ANÝZ, J. HYNEK, D. HRABĚTA, J. KRUSEOVÁ, J. ECKSCHLAGER, T. ADAM, V. ŠTĚPÁNKOVÁ, O. KIZEK, R.
Original Title
Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours
Type
journal article in Web of Science
Language
English
Original Abstract
In this study, we determined serum levels of metallothioneins (MTs) and zinc in children with solid tumours (neuroblastoma, Hodgkin lymphoma, medulloblastoma, osteosarcoma, Ewing sarcoma and nephroblastoma) by differential pulse voltammetry Brdicka reaction and ELISA. Zn(II) level in patients sera was 40% compared to controls, contrariwise, MT level was 4.2 higher in patients. No significant differences among single diagnoses were found both for Zn(II) and MT. When determined Zn(II)/MT ratio, in controls its value was 24.6, but it was 2.6 in patients. After Western-blotting with anti-MT and anti-Zn chicken antibodies, variable intensities of the bands within the samples were observed. The brightness curve obtained for each sample both for MT- and Zn blots was further analysed to produce a list of band positions together with some complementary information related to the intensity of the observed bands by the optimised algorithm.
Keywords
wavelet transformation, metallothionein, Western blot
Authors
VYSLOUŽILOVÁ, L.; KŘÍŽKOVÁ, S.; ANÝZ, J.; HYNEK, D.; HRABĚTA, J.; KRUSEOVÁ, J.; ECKSCHLAGER, T.; ADAM, V.; ŠTĚPÁNKOVÁ, O.; KIZEK, R.
RIV year
2013
Released
1. 6. 2013
ISBN
0173-0835
Periodical
Electrophoresis
Year of study
34
Number
11
State
Federal Republic of Germany
Pages from
1637
Pages to
1648
Pages count
12
BibTex
@article{BUT101161, author="Lenka {Vysloužilová} and Soňa {Křížková} and Jiří {Anýz} and David {Hynek} and Jan {Hraběta} and Jarmila {Kruseová} and Tomáš {Eckschlager} and Vojtěch {Adam} and Olga {Štěpánková} and René {Kizek}", title="Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours", journal="Electrophoresis", year="2013", volume="34", number="11", pages="1637--1648", doi="10.1002/elps.201200561", issn="0173-0835" }