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ZÍTKA, O. SKALIČKOVÁ, S. MERLOS RODRIGO, M. KREJČOVÁ, L. KOPEL, P. ADAM, V. KIZEK, R.
Original Title
Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector
Type
journal article in Web of Science
Language
English
Original Abstract
Detection of the specific sequences of pandemic-causing viral nucleic acids is area attracting numerous scientists worldwide. In this study, we focused on the isolation and detection of oligonucleotide specific sequences of life-threatening viruses (HIV (GAGCAGTGGGAATA), Influenza (TAATAACCATTGGA) and Ebola (ACCTCACTAGAAAA)) by flow injection analysis with electrochemical detection coupled with paramagnetic particles based isolation. All of used viral genomic sequences contained A6 adenine repetition on the 5 end. This modification enables us to bind them on paramagnetic particle anchoring T25 (MPs-(dT)25). The isolated sequences were subsequently hydrolysed by optimized method using microwaves to obtain single adenines. The obtained samples were detected by microfluidic tool based on stopped flow injection analysis with glassy carbon electrode in the presence of Cu(II)/or CuS nanoparticles to enhance sensitivity and to lower detection limit for adenine. Basic detection parameters as accumulation of CuS nanoparticles,flow influence and accumulation of the adenine deposition influencing the analytical response were also tested. We found that bases gave well developed signals with detection limit down to 30 ng per ml (50 pg per electrochemical cell) under the optimized conditions.
Keywords
Paramagnetic particles,viral nucleic sequences, pandemic
Authors
ZÍTKA, O.; SKALIČKOVÁ, S.; MERLOS RODRIGO, M.; KREJČOVÁ, L.; KOPEL, P.; ADAM, V.; KIZEK, R.
RIV year
2013
Released
1. 12. 2013
ISBN
1452-3981
Periodical
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Year of study
8
Number
12
State
Republic of Serbia
Pages from
12628
Pages to
12642
Pages count
15
BibTex
@article{BUT101247, author="Ondřej {Zítka} and Sylvie {Skaličková} and Miguel Ángel {Merlos Rodrigo} and Ludmila {Krejčová} and Pavel {Kopel} and Vojtěch {Adam} and René {Kizek}", title="Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector", journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE", year="2013", volume="8", number="12", pages="12628--12642", issn="1452-3981" }