Interaction of hyaluronan and CTAB studied by high resolutuon ultrasonic spectroscopy

abstract

English

High resolution ultrasonic spectroscopy (HR-US) is a new technique for material analysis based on the measurements of parameters of ultrasonic waves propagating through samples. This technique is based on the measurements of velocity and attenuation on acoustical waves at high, ultrasonic frequencies propagating through materials. This technique allows direct and non-destructive measurements without formation of derivatives or changing state of sample. Advantage of this technique is the ability of ultrasonic waves to propagate through optically non-transparent materials. The analyzed sample can be highly coloured or even opaque. In this contribution, a high-resolution ultrasonic spectroscopy technology (HR-US 102) has been employed to analyse critical aggregation concentration of the surfactant in water and in the solution with polymer. The work is focused on the study of physico-chemical interactions of hyaluronan with cationic surfactants in the different molecular weight and concentration measured using uncommon technique named High resolution ultrasound spectroscopy. Interactions in these systems are important for the design of systems for targeted delivery, especially of drugs. The experiments was be made in water and sodium chloride solution. First aim was basic study of hyaluronan in dependence on its molecular weight and on elevated temperature by measuring density and ultrasonic velocity on the densitometer DSA 5000M where we will obtain dependence density and ultrasonic velocity on the temperature and on the concentration of hyaluronan. Second aim was to determine the critical micelle concentration (CMC) and aggregation concentration (CAC) of the surfactants in the absence and presence of hyaluronan of different molecular weight by method HRUS. The interactions between very low (10-30 kDa), low (about 100 kDa) and high (about 1.5 MDa) molecular weight hyaluronan (produced by Contipro holding, Czech Republic) and cationic surfactants (CTAB - hexadecyltrimethylammonium bromide and TTAB-tetradecyltrimethylammonium bromide) will be investigated. Cationic surfactants are selected because of the negative hyaluronan charge. Ultrasound velocity and attenuation were measured. Critical aggregation concentration of CTAB in water was determined as 1 mmol/kg. Micelles were formed above CMC in water and in the solution with hyaluronan below this concentration there were not any free surfactant and above this concentration micelles were on the chain of hyaluronan.

hyaluronan, CTAB, TTAB, high resolution ultrasonic spectroscopy

KARGEROVÁ, A.; PEKAŘ, M.

2. 6. 2013

University of Oklahoma Health Sciences Center (OUHSC)

Oklahoma City, Oklahoma, USA

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