Publication detail

Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes

MERLOS RODRIGO, M. CERNEI, N. KOMÍNKOVÁ, M. ZÍTKA, O. BEKLOVÁ, M. ZEHNÁLEK, J. KIZEK, R. ADAM, V.

Original Title

Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes

Type

journal article in Web of Science

Language

English

Original Abstract

In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 ug mL 1 PC2 and 100 ug mL 1 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes

Keywords

ion exchange chromatography; mass spectrometry; MALDI-TOF; phytochelatin; cadmium; intramolecular complex

Authors

MERLOS RODRIGO, M.; CERNEI, N.; KOMÍNKOVÁ, M.; ZÍTKA, O.; BEKLOVÁ, M.; ZEHNÁLEK, J.; KIZEK, R.; ADAM, V.

RIV year

2013

Released

28. 3. 2013

Publisher

MDPI

ISBN

1660-4601

Periodical

International Journal of Environmental Research and Public Health

Year of study

10

Number

4

State

Swiss Confederation

Pages from

1304

Pages to

1311

Pages count

8

URL

http://www.mdpi.com/1660-4601/10/4/1304

Full text in the Digital Library

http://hdl.handle.net/11012/70096

BibTex

@article{BUT101373,
  author="Miguel Ángel {Merlos Rodrigo} and Natalia Vladimirovna {Cernei} and Markéta {Komínková} and Ondřej {Zítka} and Miroslava {Beklová} and Josef {Zehnálek} and René {Kizek} and Vojtěch {Adam}",
  title="Ion exchange chromatography and mass spectrometric methods for analysis of cadmium-phytochelatin (II) complexes",
  journal="International Journal of Environmental Research and Public Health",
  year="2013",
  volume="10",
  number="4",
  pages="1304--1311",
  doi="10.3390/ijerph10041304",
  issn="1660-4601",
  url="http://www.mdpi.com/1660-4601/10/4/1304"
}