Publication detail

Automatic Identification and Validation of Planar Collagen Organization in the Aorta Wall with Application to Abdominal Aortic Aneurysm

POLZER, S. GASSER, T. FORSELL, C. DRUCKMÜLLEROVÁ, H. TICHÝ, M. STAFFA, R. VLACHOVSKÝ, R. BURŠA, J.

Original Title

Automatic Identification and Validation of Planar Collagen Organization in the Aorta Wall with Application to Abdominal Aortic Aneurysm

Type

journal article - other

Language

English

Original Abstract

Arterial physiology relies on a delicate three-dimensional (3D) organization of cells and extracellular matrix, which is remarkably altered by vascular diseases like abdominal aortic aneurysms (AAA). The ability to explore the micro-histology of the aorta wall is important in the study of vascular pathologies and in the development of vascular constitutive models, i.e., mathematical descriptions of biomechanical properties of the wall. The present study reports and validates a fast image processing sequence capable of quantifying collagen fiber organization from histological stains. Powering and re-normalizing the histogram of the classical fast Fourier transformation (FFT) is a key step in the proposed analysis sequence. This modification introduces a powering parameter w, which was calibrated to best fit the reference data obtained using classical FFT and polarized light microscopy (PLM) of stained histological slices of AAA wall samples. The values of w = 3 and 7 give the best correlation (Pearson's correlation coefficient larger than 0.7, R 2 about 0.7) with the classical FFT approach and PLM measurements. A fast and operator independent method to identify collagen organization in the arterial wall was developed and validated. This overcomes severe limitations of currently applied methods like PLM to identify collagen organization in the arterial wall.

Keywords

fast Fourier Tranform, collagen organization, collagen dispersion, polarized light microscopy

Authors

POLZER, S.; GASSER, T.; FORSELL, C.; DRUCKMÜLLEROVÁ, H.; TICHÝ, M.; STAFFA, R.; VLACHOVSKÝ, R.; BURŠA, J.

RIV year

2013

Released

13. 11. 2013

Publisher

Cambridge Journals

Location

USA

ISBN

1431-9276

Periodical

MICROSCOPY AND MICROANALYSIS

Year of study

19

Number

6

State

United States of America

Pages from

1395

Pages to

1404

Pages count

10

BibTex

@article{BUT101972,
  author="Stanislav {Polzer} and Thomas Christian {Gasser} and Caroline {Forsell} and Hana {Druckmüllerová} and Michal {Tichý} and Robert {Staffa} and Robert {Vlachovský} and Jiří {Burša}",
  title="Automatic Identification and Validation of Planar Collagen Organization in the Aorta Wall with Application to Abdominal Aortic Aneurysm",
  journal="MICROSCOPY AND MICROANALYSIS",
  year="2013",
  volume="19",
  number="6",
  pages="1395--1404",
  doi="10.1017/s1431927613013251",
  issn="1431-9276"
}