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ŠMERKOVÁ, K. DOSTÁLOVÁ, S. VACULOVIČOVÁ, M. KYNICKÝ, J. TRNKOVÁ, L. KRÁLÍK, M. ADAM, V. HUBÁLEK, J. PROVAZNÍK, I. KIZEK, R.
Original Title
Investigation of interaction between magnetic silica particles andlambda phage DNA fragment
Type
journal article in Web of Science
Language
English
Original Abstract
Nucleic acids belong to the most important molecules and therefore the understanding of theirproperties, function and behavior is crucial. Even though a range of analytical and biochemicalmethods have been developed for this purpose, one common step is essential for all of them –isolation of the nucleic acid from the from complex sample matrix. The use of magnetic parti-cles for the separation of nucleic acids has many advantages over other isolation methods. In thisstudy, an isolation procedure for extraction of DNA was optimized. Each step of the isolation pro-cess including washing, immobilization and elution was optimized and therefore the efficiency wasincreased from 1.7% to 28.7% and the total time was shortened from 75 to 30 min comparing to thepreviously described method. Quantification of the particular parameter influence was performed bysquare-wave voltammetry using hanging drop mercury electrode. Further, we compared the optimizedmethod with standard chloroform extraction and applied on isolation of DNA from Staphylococcus aureusand Escherichia coli.
Keywords
DNA isolation, Magnetic particles, Lambda phage, Polymerase chain reaction, Voltammetry
Authors
ŠMERKOVÁ, K.; DOSTÁLOVÁ, S.; VACULOVIČOVÁ, M.; KYNICKÝ, J.; TRNKOVÁ, L.; KRÁLÍK, M.; ADAM, V.; HUBÁLEK, J.; PROVAZNÍK, I.; KIZEK, R.
RIV year
2013
Released
1. 12. 2013
ISBN
0731-7085
Periodical
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Year of study
86
Number
1
State
Kingdom of the Netherlands
Pages from
65
Pages to
72
Pages count
13
BibTex
@article{BUT102368, author="Kristýna {Šmerková} and Simona {Dostálová} and Markéta {Vaculovičová} and Jindřich {Kynický} and Libuše {Trnková} and Miroslav {Králík} and Vojtěch {Adam} and Jaromír {Hubálek} and Valentine {Provazník} and René {Kizek}", title="Investigation of interaction between magnetic silica particles andlambda phage DNA fragment", journal="JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS", year="2013", volume="86", number="1", pages="65--72", doi="10.1016/j.jpba.2013.07.039", issn="0731-7085" }