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OBRUČA, S. BENEŠOVÁ, P. OBORNÁ, J. MÁROVÁ, I.
Original Title
Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator
Type
journal article in Web of Science
Language
English
Original Abstract
Whole whey hydrolyzed by Alcalase (WWH) was tested as a complex nitrogen source for the production of poly(3-hydroxybutyrate) (PHB) from waste frying oils by Cupriavidus necator H16. The addition of WWH (10 % (vol/vol) of cultivation media) supported the growth of the bacterial culture and PHB accumulation; hence the PHB yields achieved in Erlenmeyer flasks were more than 3.5-fold higher than in control cultivation. The positive influence of WWH on PHB production was confirmed in experiments performed in laboratory fermentor. C. necator cultivated in the presence of WWH produced 28.1 g l-1of PHB resulting in very high product yield coefficient of 0.94 g PHB per g oil. Since PHB yields were about 40 % higher than in the control cultivation, it seems that WWH can be considered as an excellent inexpensive nitrogen source for PHB production by C. necator.
Keywords
Whole whey hydrolyzate, Cupriavidus necator, poly(3-hydroxybutyrate), complex nitrogen source, waste frying oil
Authors
OBRUČA, S.; BENEŠOVÁ, P.; OBORNÁ, J.; MÁROVÁ, I.
RIV year
2014
Released
1. 3. 2014
Publisher
Springer
Location
Nizozemí
ISBN
0141-5492
Periodical
Biotechnology Letters
Year of study
36
Number
4
State
Kingdom of the Netherlands
Pages from
775
Pages to
781
Pages count
7
BibTex
@article{BUT105505, author="Stanislav {Obruča} and Pavla {Benešová} and Jana {Oborná} and Ivana {Márová}", title="Application of protease-hydrolyzed whey as a complex nitrogen source to increase poly(3-hydroxybutyrate) production from oils by Cupriavidus necator", journal="Biotechnology Letters", year="2014", volume="36", number="4", pages="775--781", doi="10.1007/s10529-013-1407-z", issn="0141-5492" }