Publication detail

Utilization of paramagnetic microparticles for automated isolation of free circulating mRNA as a new tool in prostate cancer diagnostics

FOJTŮ, M. GUMULEC, J. BALVAN, J. RAUDENSKÁ, M. SZTALMACHOVÁ, M. POLANSKÁ, H. ŠMERKOVÁ, K. ADAM, V. KIZEK, R. MASAŘÍK, M.

Original Title

Utilization of paramagnetic microparticles for automated isolation of free circulating mRNA as a new tool in prostate cancer diagnostics

Type

journal article in Web of Science

Language

English

Original Abstract

Determination of serum mRNA gained a lot of attention in recent years, particularly from the perspective of disease markers. Streptavidin-modified paramagnetic particles (SMPs) seem an interesting technique, mainly due to possible automated isolation and high efficiency. The aim of this study was to optimize serum isolation protocol to reduce the consumption of chemicals and sample volume.

Keywords

Metallothionein, Microseparation, Paramagnetic microparticles, Prostate cancer, Serum mRNA

Authors

FOJTŮ, M.; GUMULEC, J.; BALVAN, J.; RAUDENSKÁ, M.; SZTALMACHOVÁ, M.; POLANSKÁ, H.; ŠMERKOVÁ, K.; ADAM, V.; KIZEK, R.; MASAŘÍK, M.

RIV year

2014

Released

1. 2. 2014

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

35

Number

2-3

State

Federal Republic of Germany

Pages from

306

Pages to

315

Pages count

10

BibTex

@article{BUT106913,
  author="Michaela {Fojtů} and Jaromír {Gumulec} and Jan {Balvan} and Martina {Raudenská} and Markéta {Sztalmachová} and Hana {Polanská} and Kristýna {Šmerková} and Vojtěch {Adam} and René {Kizek} and Michal {Masařík}",
  title="Utilization of paramagnetic microparticles for automated isolation of free circulating mRNA as a new tool in prostate cancer diagnostics",
  journal="Electrophoresis",
  year="2014",
  volume="35",
  number="2-3",
  pages="306--315",
  doi="10.1002/elps.201300190",
  issn="0173-0835"
}