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BLAŽKOVÁ, I. NGUYEN, H. KOMÍNKOVÁ, M. KONEČNÁ, R. CHUDOBOVÁ, D. KREJČOVÁ, L. KOPEL, P. HYNEK, D. ZÍTKA, O. BEKLOVÁ, M. ADAM, V. KIZEK, R.
Original Title
Fullerene as a transporter for doxorubicin investigated by analytical methods and in vivo imaging
Type
journal article in Web of Science
Language
English
Original Abstract
Carbon nanomaterials, including fullerenes, exhibit not only unique structure and electronic properties but also a significant potential to serve as radical scavengers and/or anti-oxidants. Their conjugation with anticancer drugs such as doxorubicin (DOX) may help to balance severe negative side effects of these cytostatics and also improve the delivery of the drug taking advantage of the enhanced cellular uptake, selectivity to cancer cells, and pH regulated release. In this study, the fullerene (C60) surface was oxidized by concentrated nitric acid, which enabled simple DOX–fullerene conjugation based on stacking and hydrophilic interactions with carboxylic groups. The strength of this noncovalent binding is pH dependent. At a low pH, the amino group of DOX is protonated, however at a higher pH, the amino group is deprotonated, resulting in stronger hydrophobic interactions with the fullerene walls. CE and HPLC were employed for characterization of resulting complexes. The cell toxicity of the conjugates was evaluated using Staphylococcus aureus and finally they were administered into the chicken embryo to assess the applicability for in vivo imaging.
Keywords
Clinical analysis, Doxorubicin, Drug delivery, Embryo, Fullerene, Nanomedicine
Authors
BLAŽKOVÁ, I.; NGUYEN, H.; KOMÍNKOVÁ, M.; KONEČNÁ, R.; CHUDOBOVÁ, D.; KREJČOVÁ, L.; KOPEL, P.; HYNEK, D.; ZÍTKA, O.; BEKLOVÁ, M.; ADAM, V.; KIZEK, R.
RIV year
2014
Released
1. 4. 2014
ISBN
0173-0835
Periodical
Electrophoresis
Year of study
35
Number
7
State
Federal Republic of Germany
Pages from
1040
Pages to
1049
Pages count
10
BibTex
@article{BUT108694, author="Iva {Blažková} and Hoai Viet {Nguyen} and Markéta {Komínková} and Romana {Konečná} and Dagmar {Hegerová} and Ludmila {Krejčová} and Pavel {Kopel} and David {Hynek} and Ondřej {Zítka} and Miroslava {Beklová} and Vojtěch {Adam} and René {Kizek}", title="Fullerene as a transporter for doxorubicin investigated by analytical methods and in vivo imaging", journal="Electrophoresis", year="2014", volume="35", number="7", pages="1040--1049", doi="10.1002/elps.201300393", issn="0173-0835" }