Publication detail

Plasmid HIV p24 Gene Detection on Mercury Film Electrode using Osmium Labelling

KUDR, J. NEJDL, L. SKALIČKOVÁ, S. RUTTKAY-NEDECKÝ, B. MERLOS RODRIGO, M. DOSTÁLOVÁ, S. JIMENEZ JIMENEZ, A. CHUDOBOVÁ, D. ČÍHALOVÁ, K. NOVOTNÁ, M. KOPEL, P. KYNICKÝ, J. ADAM, V. KIZEK, R.

Original Title

Plasmid HIV p24 Gene Detection on Mercury Film Electrode using Osmium Labelling

Type

journal article in Web of Science

Language

English

Original Abstract

The early detection of HIV-positive individuals before infecting healthy individuals is the basic principle to fight against AIDS. Although a lot of methods has been developed to detect HIV, electrochemistry is a promising tool thanks to its high sensitivity, low cost, ease of use and mostly no antibody-based detection.The aim of our study was to perform the electrochemical detection of HIV p24 gene for capsid protein. We utilized the ability of osmium tetroxide bipyridyl (OsO4(bpy)) to create the complex with thymine and used it to label the oligonucleotide complementary to the p24. We also used the thiolated oligonucleotide probe to anchor the labelled p24 gene to the magnetic particle, which served to isolate the construct from the solution of non-anchored molecules. The detection of p24 gene was performed on the mercury film glassy-carbon electrode by the adsorptive transfer method using difference pulse voltammetry. The best mercury film on the surface of glassy carbon working electrode was received using KNO3 as supporting electrolyte and by the set of potential -0.9 V for 300 s. We showed that also the femtomole

Keywords

Electrochemistry; Glassy carbon electrode; Gel electrophoresis; p24 gene

Authors

KUDR, J.; NEJDL, L.; SKALIČKOVÁ, S.; RUTTKAY-NEDECKÝ, B.; MERLOS RODRIGO, M.; DOSTÁLOVÁ, S.; JIMENEZ JIMENEZ, A.; CHUDOBOVÁ, D.; ČÍHALOVÁ, K.; NOVOTNÁ, M.; KOPEL, P.; KYNICKÝ, J.; ADAM, V.; KIZEK, R.

RIV year

2014

Released

1. 7. 2014

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

9

Number

7

State

Republic of Serbia

Pages from

3409

Pages to

3418

Pages count

10

BibTex

@article{BUT109369,
  author="Jiří {Kudr} and Lukáš {Nejdl} and Sylvie {Skaličková} and Branislav {Ruttkay-Nedecký} and Miguel Ángel {Merlos Rodrigo} and Simona {Dostálová} and Ana Maria {Jimenez Jimenez} and Dagmar {Hegerová} and Kristýna {Číhalová} and Marie {Novotná} and Pavel {Kopel} and Jindřich {Kynický} and Vojtěch {Adam} and René {Kizek}",
  title="Plasmid HIV p24 Gene Detection on Mercury Film Electrode using Osmium Labelling",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2014",
  volume="9",
  number="7",
  pages="3409--3418",
  issn="1452-3981"
}