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ZIKMUND, T. KVASNICA, L. TÝČ, M. KŘÍŽOVÁ, A. ČOLLÁKOVÁ, J. CHMELÍK, R.
Original Title
Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy
Type
journal article in Web of Science
Language
English
Original Abstract
Transmitted light holographic microscopy is particularly used for quantitative phase imaging of transparent microscopic objects such as living cells. The study of the cell is based on extraction of the dynamic data on cell behaviour from the time-lapse sequence of the phase images. However, the phase images are affected by the phase aberrations that make the analysis particularly difficult. This is because the phase deformation is prone to change during long-term experiments. Here, we present a novel algorithm for sequential processing of living cells phase images in a time-lapse sequence. The algorithm compensates for the deformation of a phase image using weighted least-squares surface fitting. Moreover, it identifies and segments the individual cells in the phase image.All these procedures are performed automatically and applied immediately after obtaining every single phase image. This property of the algorithm is important for real-time cell quantitative phase imaging and instantaneous control of the course of the experiment by playback of the recorded sequence up to actual time. Such operators intervention is a forerunner of process automation derived from image analysis. The efficiency of the propounded algorithm is demonstrated on images of rat fibrosarcoma cells using an off-axis holographic microscope.
Keywords
Aberration compensation, cell tracking, digital image processing, quantitative phase imaging, real-time holography.
Authors
ZIKMUND, T.; KVASNICA, L.; TÝČ, M.; KŘÍŽOVÁ, A.; ČOLLÁKOVÁ, J.; CHMELÍK, R.
RIV year
2014
Released
1. 11. 2014
ISBN
0022-2720
Periodical
Journal of Microscopy
Year of study
256
Number
2
State
United Kingdom of Great Britain and Northern Ireland
Pages from
117
Pages to
125
Pages count
9
BibTex
@article{BUT109829, author="Tomáš {Zikmund} and Lukáš {Kvasnica} and Matěj {Týč} and Aneta {Křížová} and Jana {Čolláková} and Radim {Chmelík}", title="Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy", journal="Journal of Microscopy", year="2014", volume="256", number="2", pages="117--125", doi="10.1111/jmi.12165", issn="0022-2720" }