Publication detail

Characterization of Egg Laying Hen and Broiler Fecal Microbiota in Poultry Farms in Croatia, Czech Republic, Hungary and Slovenia

VÍDEŇSKÁ, P. RAHMAN, M. FALDYNOVÁ, M. BABÁK, V. MATULOVÁ, M. PRUKNER-RADOVCIC, E. KŘÍŽEK, I. SMOLE-MOZINA, S. KOVAC, J. SZMOLKA, A. NAGY, B. SEDLÁŘ, K. ČEJKOVÁ, D. RYCHLÍK, I.

Original Title

Characterization of Egg Laying Hen and Broiler Fecal Microbiota in Poultry Farms in Croatia, Czech Republic, Hungary and Slovenia

Type

journal article in Web of Science

Language

English

Original Abstract

Poultry meat is the most common protein source of animal origin for humans. However, intensive breeding of animals in confined spaces has led to poultry colonisation by microbiota with a zoonotic potential or encoding antibiotic resistances. In this study we were therefore interested in the prevalence of selected antibiotic resistance genes and microbiota composition in feces of egg laying hens and broilers originating from 4 different Central European countries determined by real-time PCR and 16S rRNA gene pyrosequencing, respectively. strA gene was present in 1 out of 10,000 bacteria. The prevalence of sul1, sul2 and tet(B) in poultry microbiota was approx. 6 times lower than that of the strA gene. tet(A) and cat were the least prevalent being present in around 3 out of 10,000,000 bacteria forming fecal microbiome. The core chicken fecal microbiota was formed by 26 different families. Rather unexpectedly, representatives of Desulfovibrionaceae and Campylobacteraceae, both capable of hydrogen utilisation in complex microbial communities, belonged among core microbiota families. Understanding the roles of individual population members in the total metabolism of the complex community may allow for interventions which might result in the replacement of Campylobacteraceae with Desulfovibrionaceae and a reduction of Campylobacter colonisation in broilers, carcasses, and consequently poultry meat products.

Keywords

metagenomics, chicken microbiota, 16S rRNA

Authors

VÍDEŇSKÁ, P.; RAHMAN, M.; FALDYNOVÁ, M.; BABÁK, V.; MATULOVÁ, M.; PRUKNER-RADOVCIC, E.; KŘÍŽEK, I.; SMOLE-MOZINA, S.; KOVAC, J.; SZMOLKA, A.; NAGY, B.; SEDLÁŘ, K.; ČEJKOVÁ, D.; RYCHLÍK, I.

RIV year

2014

Released

16. 10. 2014

Publisher

Public Library of Science

Location

California (US)

ISBN

1932-6203

Periodical

PLOS ONE

Year of study

9

Number

10

State

United States of America

Pages from

e110076

Pages to

e110076

Pages count

8

URL

http://europepmc.org/articles/PMC4199679?pdf=render

Full text in the Digital Library

http://hdl.handle.net/11012/69201