Publication detail

17B-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line

HEGER, Z. GUMULEC, J. CERNEI, N. TMEJOVÁ, K. KOPEL, P. BALVAN, J. MASAŘÍK, M. ZÍTKA, O. BEKLOVÁ, M. ADAM, V. KIZEK, R.

Original Title

17B-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line

Type

journal article in Web of Science

Language

English

Original Abstract

The present study suggests and describes theapplication of a delivery system for antisense oligonucleotides against mRNA encoding estrogen receptor proteins alfa and beta. The delivery system is composed of a cationic liposome envelope containing 17B-estradiol (E2) in its structure. Cationic liposomes protect cargo against the extracellular matrix, and E2 can increase its shuttling efficiency into cells. Using MCF-7 cells derived from estrogen receptor-positive ductal carcinoma, treatment with liposomes against ERa was found to decrease MCF-7 proliferation, and importantly the application of both the antisense against ERa and B exhibited an antiproliferative effect expressed as cell viability. Using qRT-PCR, it was shown that MT1A, NF-KB1 and K-ras genes, but not TFF1, were downregulated using E2-based liposomes (evaluated at P=0.05). Further indicators of oxidative stress were employed to assess the effect on treatment efficiency. Glutathione (GSH/ GSSG redox ratio), metallothionein (MT) and malondialdehyde (MDA) confirmed a positive effect of antisense therapy resulting in their decreased levels in the MCF-7 cells. Based on these data, we suggest that E2-based liposomes offer sufficient transfer efficiency and moreover, due to the effect on NF-KB1, MT and GSH, tumor cells can be chemosensitized to increase treatment effectiveness.

Keywords

antisense therapy, delivery, glutathione, liposome, malondialdehyde, metallothionein

Authors

HEGER, Z.; GUMULEC, J.; CERNEI, N.; TMEJOVÁ, K.; KOPEL, P.; BALVAN, J.; MASAŘÍK, M.; ZÍTKA, O.; BEKLOVÁ, M.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

1. 2. 2015

ISBN

1021-335X

Periodical

ONCOLOGY REPORTS

Year of study

33

Number

2

State

Hellenic Republic

Pages from

921

Pages to

929

Pages count

9

BibTex

@article{BUT110383,
  author="Zbyněk {Heger} and Jaromír {Gumulec} and Natalia Vladimirovna {Cernei} and Kateřina {Tmejová} and Pavel {Kopel} and Jan {Balvan} and Michal {Masařík} and Ondřej {Zítka} and Miroslava {Beklová} and Vojtěch {Adam} and René {Kizek}",
  title="17B-estradiol-containing liposomes as a novel delivery system for the antisense therapy of ER-positive breast cancer: An in vitro study on the MCF-7 cell line",
  journal="ONCOLOGY REPORTS",
  year="2015",
  volume="33",
  number="2",
  pages="921--929",
  doi="10.3892/or.2014.3627",
  issn="1021-335X"
}