Publication detail

Interactions between CdTe quantum dots and DNA revealed by capillary electrophoresis with laser-induced fluorescence detection

STANISAVLJEVIC, M. PEKÁRKOVÁ, J. DOSTÁLOVÁ, S. KŘÍŽKOVÁ, S. VACULOVIČOVÁ, M. ADAM, V. KIZEK, R.

Original Title

Interactions between CdTe quantum dots and DNA revealed by capillary electrophoresis with laser-induced fluorescence detection

Type

journal article in Web of Science

Language

English

Original Abstract

Quantum dots (QDs) are one of the most promising nanomaterials, due to their size-dependent characteristics as well as easily controllable size during the synthesis process. They are promising label material and their interaction with biomolecules is of great interest for science. In this study, CdTe QDs were synthesized under optimal conditions for 2 nm size. Characterization and verification of QDs synthesis procedure were done by fluorimetric method and with CE. Afterwards, QDs interaction with chicken genomic DNA and 500 bpDNA fragment was observed employing CE-LIF and gel electrophoresis. Performed interaction relies on possible matching between size of QDs and major groove of the DNA, which is approximately 2.1 nm.

Keywords

Bacteriophage; DNA; Interaction; Quantum dots

Authors

STANISAVLJEVIC, M.; PEKÁRKOVÁ, J.; DOSTÁLOVÁ, S.; KŘÍŽKOVÁ, S.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

RIV year

2014

Released

1. 8. 2014

Publisher

WILEY-BLACKWELL

Location

111 RIVER ST, HOBOKEN 07030-5774, NJ USA

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

35

Number

18

State

Federal Republic of Germany

Pages from

2587

Pages to

2592

Pages count

6

BibTex

@article{BUT110806,
  author="Maja {Stanisavljevic} and Jana {Pekárková} and Simona {Dostálová} and Soňa {Křížková} and Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="Interactions between CdTe quantum dots and DNA revealed by capillary electrophoresis with laser-induced fluorescence detection",
  journal="Electrophoresis",
  year="2014",
  volume="35",
  number="18",
  pages="2587--2592",
  doi="10.1002/elps.201400204",
  issn="0173-0835"
}