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KOMÍNKOVÁ, M. MICHÁLEK, P. GURÁŇ, R. CERNEI, N. RUTTKAY-NEDECKÝ, B. ANÝZ, J. ZÍTKA, O. ŠTĚPÁNKOVÁ, O. PIKULA, J. ADAM, V. BEKLOVÁ, M. KIZEK, R.
Original Title
From Amino Acids Profile to Protein Identification: Searching for Differences in Roe Deer Papilloma
Type
journal article in Web of Science
Language
English
Original Abstract
Papillomaviruses, small non-enveloped DNA viruses, are considered as the cause of a number of cancers, such as a cervical, skin, anal, and penile cancers. Roe deer papillomavirus infection can be easily characterized by typical warts on the skin. The aim of this study was to characterize differences of amino acid and protein composition in healthy and tumour tissue of roe deer using methods including ion-exchange chromatography, SDS-PAG E, two-dimensional gel electrophoresis and MALDI-TOF MS. The obtained data were statistically evaluated. Cluster analysis of all variables showed biggest differences in proline in control and papilloma samples. Further, we aimed at electrophoretic analysis of the samples. Discriminant analysis projections to latent structures (Projection to the latent structure Discrimination analysis, syn. Discrimination Partial least squares analysis, PLS DA) was used to evaluate the obtained results. Examining model PLS DA, there was found that the greatest effect on whether the individual is classified as healthy, or with papilloma, is the latent variable that best correlates with the molecular mass from 108.8 to 128.1 kDa and from 231.2 to 290.4 kDa in the original signal. Finally, using mass spectrometry three proteins, pyridoxal kinase, myoblast determination protein and leucine zipper transcription factor-like protein 1, most likely related to cancer were found.
Keywords
Electrophoresis, Ion-exchange chromatography, Papillomavirus, Roe deer , Protein fractions, Amino acids profile
Authors
KOMÍNKOVÁ, M.; MICHÁLEK, P.; GURÁŇ, R.; CERNEI, N.; RUTTKAY-NEDECKÝ, B.; ANÝZ, J.; ZÍTKA, O.; ŠTĚPÁNKOVÁ, O.; PIKULA, J.; ADAM, V.; BEKLOVÁ, M.; KIZEK, R.
RIV year
2014
Released
1. 4. 2014
ISBN
0009-5893
Periodical
CHROMATOGRAPHIA
Year of study
77
Number
7-8
State
Federal Republic of Germany
Pages from
609
Pages to
617
Pages count
9
BibTex
@article{BUT110908, author="Markéta {Komínková} and Petr {Michálek} and Roman {Guráň} and Natalia Vladimirovna {Cernei} and Branislav {Ruttkay-Nedecký} and Jiří {Anýz} and Ondřej {Zítka} and Olga {Štěpánková} and Jiří {Pikula} and Vojtěch {Adam} and Miroslava {Beklová} and René {Kizek}", title="From Amino Acids Profile to Protein Identification: Searching for Differences in Roe Deer Papilloma", journal="CHROMATOGRAPHIA", year="2014", volume="77", number="7-8", pages="609--617", doi="10.1007/s10337-014-2658-0", issn="0009-5893" }