Publication detail

CAPILLARY LIQUID CHROMATOGRAPHY: GREEN ANALYTICAL TECHNIQUE AND SOME SIMPLE WAYS OF ITS REALIZATION

ŠESTÁK, J. KAHLE, V.

Original Title

CAPILLARY LIQUID CHROMATOGRAPHY: GREEN ANALYTICAL TECHNIQUE AND SOME SIMPLE WAYS OF ITS REALIZATION

Type

abstract

Language

English

Original Abstract

Reduction of organic solvents consumption as well as replacement of some solvents with less harmful alternatives is required by the green chemistry philosophy. High performance liquid chromatography (HPLC) is a widely used tool in analytical chemistry. Hence, in the green analytical chemistry, a solvent consumption of new liquid chromatography methods should be reduced substantially. Realization of the HPLC with capillary columns thus represents a straightforward option of the significant solvent consumption decrease. Namely, N-fold reduction of the internal column diameter leads to N2-fold reduction of the column cross section and the corresponding flow rate as well. In this presentation, some equations related to the column diameter reduction will be highlighted and resulting demands on the sample injection volume and the photometric detection will be discussed. Acetonitrile is a common organic modifier in HPLC mobile phases due to UV transparency, low viscosity and low reactivity. However, the green chemistry prefers usage of methanol or even ethanol or acetone as environmentally more friendly solvents. Some properties of these solvents will be discussed as well. Finally, some simple options of the realization of isocratic and gradient capillary liquid chromatography are presented.

Keywords

capillary columns; green analytical chemistry; sipmple systems

Authors

ŠESTÁK, J.; KAHLE, V.

Released

3. 12. 2014

Publisher

Brno University of Technology

Location

Brno, Czech Republic

ISBN

978-80-214-5073-8

Book

Book of Abstracts The 15th meeting on environmental chemistry, Brno, Czech Republic, December 3 - 6, 2014

Pages from

41

Pages to

41

Pages count

1

BibTex

@misc{BUT110988,
  author="Jozef {Šesták} and Vladislav {Kahle}",
  title="CAPILLARY LIQUID CHROMATOGRAPHY: GREEN ANALYTICAL TECHNIQUE AND SOME SIMPLE WAYS OF ITS REALIZATION",
  booktitle="Book of Abstracts
The 15th meeting on environmental chemistry, Brno, Czech Republic, December 3 - 6, 2014",
  year="2014",
  pages="41--41",
  publisher="Brno University of Technology",
  address="Brno, Czech Republic",
  isbn="978-80-214-5073-8",
  note="abstract"
}