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ADAM, V. CHUDOBOVÁ, D. TMEJOVÁ, K. ČÍHALOVÁ, K. KŘÍŽKOVÁ, S. GURÁŇ, R. KOMÍNKOVÁ, M. ŽŮREK, M. KREMPLOVÁ, M. JIMENEZ JIMENEZ, A. NOVOTNÁ, M. HYNEK, D. PEKAŘÍK, V. KIZEK, R.
Original Title
An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry
Type
journal article in Web of Science
Language
English
Original Abstract
This study was focused on the application of electrochemical methods for studying of bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after theapplication of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 M). Bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like modelorganisms for studying of metals influence to metallothionein expression. Metallothionein was isolatedusing fast protein liquid chromatography and quantified by electrochemical methods. The occurrenceof metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15(MT dimer) and 22 kDa (MT trimer).
Keywords
Escherichia coli, Metallothionein, Voltammetry, Cloning, Metal Ionsa
Authors
ADAM, V.; CHUDOBOVÁ, D.; TMEJOVÁ, K.; ČÍHALOVÁ, K.; KŘÍŽKOVÁ, S.; GURÁŇ, R.; KOMÍNKOVÁ, M.; ŽŮREK, M.; KREMPLOVÁ, M.; JIMENEZ JIMENEZ, A.; NOVOTNÁ, M.; HYNEK, D.; PEKAŘÍK, V.; KIZEK, R.
RIV year
2014
Released
10. 9. 2014
ISBN
0013-4686
Periodical
Electrochimica Acta
Year of study
140
Number
SI
State
United Kingdom of Great Britain and Northern Ireland
Pages from
11
Pages to
19
Pages count
9
BibTex
@article{BUT111094, author="Vojtěch {Adam} and Dagmar {Hegerová} and Kateřina {Tmejová} and Kristýna {Číhalová} and Soňa {Křížková} and Roman {Guráň} and Markéta {Komínková} and Michal {Žůrek} and Monika {Kremplová} and Ana Maria {Jimenez Jimenez} and Marie {Novotná} and David {Hynek} and Vladimír {Pekařík} and René {Kizek}", title="An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry", journal="Electrochimica Acta", year="2014", volume="140", number="SI", pages="11--19", doi="10.1016/j.electacta.2014.06.091", issn="0013-4686" }