Publication detail

Preconcentration based on paramagnetic microparticles for the separation of sarcosine using hydrophilic interaction liquid chromatography coupled with coulometric detection

ZÍTKA, O. HEGER, Z. KOMÍNKOVÁ, M. SKALIČKOVÁ, S. KŘÍŽKOVÁ, S. ADAM, V. KIZEK, R.

Original Title

Preconcentration based on paramagnetic microparticles for the separation of sarcosine using hydrophilic interaction liquid chromatography coupled with coulometric detection

Type

journal article in Web of Science

Language

English

Original Abstract

Sarcosine has been identified as a potential prostate cancer marker. To provide determination of this compound, a number of methods are developing. In this study, we optimized a method for its separation by hydrophilic interaction LC with electrochemical detection (ED). Due to the fact that mobile phases commonly used for this type of separation altered the LODs measured by electrochemical detectors, we applied postcolumn dosing of buffer suitable for ED. The optimized conditions were mobile phase A acetonitrile, mobile phase B water in the ratio A/B 70:30, with postcolumn addition of mobile phase C (200 mM phosphate bufferpH9). The optimal mixing ratio wasA+B/C 1:1 with a flow rate of 0.80 mL/min (0.40 + 0.40 mL/min) and detection potential of 1000 mV. Due to the optimization of the parameters for effective separation, which had to meet the optimal parameters of ED, we reached a good resolution for separation also with a good LOD (100 nM). In addition, we successfully carried out sarcosine analysis bound on our modified paramagnetic microparticles with the ability to preconcentrate sarcosine isolated from artificial urine.

Keywords

Paramagnetic separation, Prostate cancer, Sarcosine, Urinary metabolites

Authors

ZÍTKA, O.; HEGER, Z.; KOMÍNKOVÁ, M.; SKALIČKOVÁ, S.; KŘÍŽKOVÁ, S.; ADAM, V.; KIZEK, R.

RIV year

2014

Released

1. 3. 2014

ISBN

1615-9306

Periodical

Journal of Separation Science

Year of study

37

Number

5

State

Federal Republic of Germany

Pages from

465

Pages to

475

Pages count

11

BibTex

@article{BUT111194,
  author="Ondřej {Zítka} and Zbyněk {Heger} and Markéta {Komínková} and Sylvie {Skaličková} and Soňa {Křížková} and Vojtěch {Adam} and René {Kizek}",
  title="Preconcentration based on paramagnetic microparticles for the separation of sarcosine using hydrophilic interaction liquid chromatography coupled with coulometric detection",
  journal="Journal of Separation Science",
  year="2014",
  volume="37",
  number="5",
  pages="465--475",
  doi="10.1002/jssc.201301188",
  issn="1615-9306"
}