Publication detail

Study of metallothionein–quantum dots interactions

TMEJOVÁ, K. HYNEK, D. KOPEL, P. KŘÍŽKOVÁ, S. TRNKOVÁ, L. ADAM, V. KIZEK, R.

Original Title

Study of metallothionein–quantum dots interactions

Type

journal article in Web of Science

Language

English

Original Abstract

Nanoparticles have gained increasing interest in medical and in vivo applications. Metallothionein (MT)is well known as a maintainer of metal ions balance in intracellular space. This is due to high affinityof this protein to any reactive species including metals and reactive oxygen species. The purpose ofthis study was to determine the metallothionein–quantum dots interactions that were investigated byspectral and electrochemical techniques. CuS, CdS, PbS, and CdTe quantum dots (QDs) were analysed.The highest intensity was shown for CdTe, than for CdS measured by fluorescence. These results weresupported by statistical analysis and considered as significant. Further, these interactions were analysedusing gel electrophoresis, where MT aggregates forming after interactions with QDs were detected. Usingdifferential pulse voltammetry Brdicka reaction, QDs and MT were studied. This method allowed us toconfirm spectral results and, moreover, to observe the changes in MT structure causing new voltammetricpeaks called X and Y, which enhanced with the prolonged time of interaction up to 6 h.

Keywords

Electrochemical detection, Metallothionein, Quantum dots, Interaction

Authors

TMEJOVÁ, K.; HYNEK, D.; KOPEL, P.; KŘÍŽKOVÁ, S.; TRNKOVÁ, L.; ADAM, V.; KIZEK, R.

RIV year

2014

Released

1. 5. 2014

ISBN

0927-7765

Periodical

Colloids and Surfaces B: Biointerfaces

Year of study

117

Number

1

State

Kingdom of the Netherlands

Pages from

534

Pages to

537

Pages count

4

BibTex

@article{BUT112398,
  author="Kateřina {Tmejová} and David {Hynek} and Pavel {Kopel} and Soňa {Křížková} and Libuše {Trnková} and Vojtěch {Adam} and René {Kizek}",
  title="Study of metallothionein–quantum dots interactions",
  journal="Colloids and Surfaces B: Biointerfaces",
  year="2014",
  volume="117",
  number="1",
  pages="534--537",
  doi="10.1016/j.colsurfb.2014.03.013",
  issn="0927-7765"
}