Publication detail

The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom

NGUYEN, H. HEGER, Z. KOMÍNKOVÁ, M. MICHÁLEK, P. GUMULEC, J. GURÁŇ, R. PŘIDAL, A. FERNANDEZ, C. HYNEK, D. ADAM, V. KIZEK, R.

Original Title

The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom

Type

journal article in Web of Science

Language

English

Original Abstract

We present in this manuscript for the first time the electrochemical and statistical evaluation of FPLC isolation of mellitin and apamin from honey bee (Apis mellifera) venom. Venoms are extremely complex blends of diverse substances that target a myriad of receptors or ion channels. Therefore, toxins, isolated from venomous organisms can be a valuable tool with diverse biological applications. In this study we decided to optimize the purification of honey bee venom by using fast protein liquid chromatography, to obtain biologically active peptide - melittin (2846.46 Da). Due to a presence of other compounds with similar molecular weight (apamin 2027.34 Da), we optimized a differential pulse voltammetry method with adsorptive transfer technique (AdT DPV), utilizing Brdicka supporting electrolyte for measurements. Typical voltammograms - fingerprints for each substance were obtained and numerical projections of voltammograms were employed to propose an artificial neural network. Our suggested neural network can simply predict the content of each peptide in fraction with following performance: 100 % for training and 100 % for testing.

Keywords

Antimicrobial peptides; Apis mellifera; Brdicka reaction; MALDI-TOF; Differential pulse voltammetry

Authors

NGUYEN, H.; HEGER, Z.; KOMÍNKOVÁ, M.; MICHÁLEK, P.; GUMULEC, J.; GURÁŇ, R.; PŘIDAL, A.; FERNANDEZ, C.; HYNEK, D.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

1. 2. 2015

Publisher

ESG

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

10

Number

2

State

Republic of Serbia

Pages from

1249

Pages to

1260

Pages count

12

URL

http://www.electrochemsci.org/papers/vol10/100201249.pdf

Full text in the Digital Library

http://hdl.handle.net/11012/201450

BibTex

@article{BUT114202,
  author="Hoai Viet {Nguyen} and Zbyněk {Heger} and Markéta {Komínková} and Petr {Michálek} and Jaromír {Gumulec} and Roman {Guráň} and Antonín {Přidal} and Carlos {Fernandez} and David {Hynek} and Vojtěch {Adam} and René {Kizek}",
  title="The Electrochemical and Statistical Evaluation of Isolation of Mellitin and Apamin from Honey Bee (Apis Mellifera) Venom",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2015",
  volume="10",
  number="2",
  pages="1249--1260",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/100201249.pdf"
}