Publication detail

Automatic Evaluation of Collagen Fiber Directions from Polarized Light Microscopy Images

NOVÁK, K. POLZER, S. TICHÝ,M. BURŠA, J.

Original Title

Automatic Evaluation of Collagen Fiber Directions from Polarized Light Microscopy Images

Type

journal article in Web of Science

Language

English

Original Abstract

Mechanical properties of the arterial wall depend largely on orientation and density of collagen fiber bundles. Several methods have been developed for observation of collagen orientation and density; the most frequently applied collagen-specific manual approach is based on polarized light (PL). However, it is very time consuming and the results are operator dependent. We have proposed a new automated method for evaluation of collagen fiber direction from two-dimensional polarized light microscopy images (2D PLM). The algorithm has been verified against artificial images and validated against manual measurements. Finally the collagen content has been estimated. The proposed algorithm was capable of estimating orientation of some 35 k points in 15 min when applied to aortic tissue and over 500 k points in 35 min for Achilles tendon. The average angular disagreement between each operator and the algorithm was -9.3±8.6° and -3.8±8.6° in the case of aortic tissue and -1.6±6.4° and 2.6±7.8° for Achilles tendon. Estimated mean collagen content was 30.3±5.8% and 94.3±2.7% for aortic media and Achilles tendon, respectively. The proposed automated approach is operator independent and several orders faster than manual measurements and therefore has the potential to replace manual measurements of collagen orientation via PLM.

Keywords

collagen fiber orientation; image analysis; phase correlation; polarized light microscopy

Authors

NOVÁK, K.; POLZER, S.; TICHÝ,M.; BURŠA, J.

RIV year

2015

Released

8. 5. 2015

Publisher

Cambridge Journals

Location

USA

ISBN

1431-9276

Periodical

MICROSCOPY AND MICROANALYSIS

Year of study

21

Number

4

State

United States of America

Pages from

863

Pages to

875

Pages count

13

URL

http://journals.cambridge.org/repo_S962LC6D3IMqvA

BibTex

@article{BUT114756,
  author="Kamil {Novák} and Stanislav {Polzer} and Jiří {Burša} and Michal {Tichý}",
  title="Automatic Evaluation of Collagen Fiber Directions from Polarized Light Microscopy Images",
  journal="MICROSCOPY AND MICROANALYSIS",
  year="2015",
  volume="21",
  number="4",
  pages="863--875",
  doi="10.1017/S1431927615000586",
  issn="1431-9276",
  url="http://journals.cambridge.org/repo_S962LC6D3IMqvA"
}