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BAIAZITOVA, L. SVOBODA, O. ČMIEL, V. PROVAZNÍK, I. FOHLEROVÁ, Z. HUBÁLEK, J.
Original Title
The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency
Type
conference paper
Language
English
Original Abstract
In this work the chemical (polyethylenimine) and magnetic nanoparticle (MATRA) based transfection methods of channelrhodopsin-2 to HEK293 cell has been compared. We optimized transfection protocol by changing reagent and DNA amounts and the most appropriate fluorescent results were obtained after 24 hours of incubation from transfection time.
Keywords
HEK293 cells, transfection, channelrhodopsin-2, polyethylenimine, MATRA-A reagent
Authors
BAIAZITOVA, L.; SVOBODA, O.; ČMIEL, V.; PROVAZNÍK, I.; FOHLEROVÁ, Z.; HUBÁLEK, J.
RIV year
2015
Released
26. 5. 2015
Publisher
Masarykova Univerzita - MUNI PRESS
Location
Brno, Czech Republic
ISBN
978-80-210-7857-4
Book
XV. Workshop of Physical Chemists and Electrochemists
Edition
1
Edition number
NEUVEDENO
Pages from
71
Pages to
73
Pages count
3
BibTex
@inproceedings{BUT115179, author="Larisa {Chmelíková} and Ondřej {Svoboda} and Vratislav {Čmiel} and Valentine {Provazník} and Zdenka {Fohlerová} and Jaromír {Hubálek}", title="The comparison of chemical and magnetic channelrhodopsin-2 transfection efficiency", booktitle="XV. Workshop of Physical Chemists and Electrochemists", year="2015", series="1", number="1", pages="71--73", publisher="Masarykova Univerzita - MUNI PRESS", address="Brno, Czech Republic", isbn="978-80-210-7857-4" }