Publication detail

Doubling Throughput of a Real-Time PCR

AHRBERG, C. NEUŽIL, P.

Original Title

Doubling Throughput of a Real-Time PCR

Type

journal article in Web of Science

Language

English

Original Abstract

We added an intercallating dye to probe-based real-time PCR. One set of oligonucleotides had FAM probe while the second one was without. We were able to extract two DNA amplification curves using a single fluorescence channel

Keywords

real-time PCR, FAM probe, intercallating dye

Authors

AHRBERG, C.; NEUŽIL, P.

RIV year

2015

Released

10. 7. 2015

Publisher

Macmillan Publishers

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

15

Number

10

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

10

Pages count

10

URL

http://www.nature.com/articles/srep12595

Full text in the Digital Library

http://hdl.handle.net/11012/61769

BibTex

@article{BUT115302,
  author="Christian D. {Ahrberg} and Pavel {Neužil}",
  title="Doubling Throughput of a Real-Time PCR",
  journal="Scientific Reports",
  year="2015",
  volume="15",
  number="10",
  pages="1--10",
  doi="10.1038/srep12595",
  issn="2045-2322",
  url="http://www.nature.com/articles/srep12595"
}