Publication detail

Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation

KŘÍŽKOVÁ, S. NGUYEN, H. STANISAVLJEVIC, M. KOPEL, P. VACULOVIČOVÁ, M. ADAM, V. KIZEK, R.

Original Title

Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation

Type

book chapter

Language

English

Original Abstract

The emergence of drug-resistant bacteria and new or changing infectious pathogens is an important public health problem as well as a serious socioeconomic concern. Immunomagnetic separation-based methods create new possibilities for rapidly recognizing many of these pathogens. Nanomaterial-based techniques including fl uorescent labeling by quantum dots as well as immunoextraction by magnetic particles are excellent tools for such purposes. Moreover, the combination with capillary electrophoresis in miniaturized microchip arrangement brings numerous benefi ts such as fast and rapid analysis, low sample consumption, very sensitive electrochemical and fl uorescent detection, portable miniaturized instrumentation, and rapid and inexpensive device fabrication. Here the use of superparamagnetic particle-based fully automated instrumentation to isolate pathogen Staphylococcus aureus and its Zn(II)-containing proteins (Zn-proteins) is reported using a robotic pipetting system speeding up the sample preparation and enabling to analyze 48 real samples within 6 h. Cell lysis and Zn-protein extractions were obtained from a minimum of 100 cells with the suffi cient yield for SDS-PAGE (several tens ng of proteins).

Keywords

Magnetic particles , Immunoseparation , Staphylococcus aureus , Zn-protein

Authors

KŘÍŽKOVÁ, S.; NGUYEN, H.; STANISAVLJEVIC, M.; KOPEL, P.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

1. 7. 2015

Publisher

Springer Science+Business Media New York 2015

ISBN

978-1-4939-2353-3

Book

Microchip Capillary Electrophoresis Protocols

Edition number

1274

Pages from

67

Pages to

79

Pages count

13

BibTex

@inbook{BUT115342,
  author="Soňa {Křížková} and Hoai Viet {Nguyen} and Maja {Stanisavljevic} and Pavel {Kopel} and Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="Microchip Capillary Electrophoresis: Quantum Dots and Paramagnetic Particles for Bacteria Immunoseparation",
  booktitle="Microchip Capillary Electrophoresis Protocols",
  year="2015",
  publisher="Springer Science+Business Media New York 2015",
  edition="1274",
  pages="67--79",
  doi="10.1007/978-1-4939-2353-3\{_}6,",
  isbn="978-1-4939-2353-3"
}