Publication detail

Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs

KOMÍNKOVÁ, M. KIZEK, R. MICHÁLEK, P. ČÍHALOVÁ, K. GURÁŇ, R. CERNEI, N. NEJDL, L. ŠMERKOVÁ, K. DOSTÁLOVÁ, S. CHUDOBOVA, D. HEGER, Z. VESELÝ, R. GUMULEC, J. KYNICKÝ, J. XHAXHIU, K. ZÍTKA, O. ADAM, V.

Original Title

Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs

Type

journal article in Web of Science

Language

English

Original Abstract

In this work, we focused on the differences between bacterial cultures of E. coli obtained from swabs of infectious wounds of patients compared to laboratory E. coli. In addition, blocking of the protein responsible for the synthesis of glutathione (γ-glutamylcysteine synthase—GCL) using 10 mM buthionine sulfoximine was investigated. Each E. coli showed significant differences in resistance to antibiotics. According to the etermined resistance, E. coli were divided into experimental groups based on a statistical evaluation of their properties as more resistant and more sensitive. These groups were also used for finding the differences in a dependence of the glutathione pathway on resistance to antibiotics. More sensitive E. coli showed the same kinetics of glutathione synthesis while blocking GCL (Km 0.1 μM), as compared to non-blocking. In addition, the most frequent mutations in genes of glutathione synthetase, glutathione peroxidase and glutathione reductase were observed in this group compared to laboratory E.coli. The group of “more resistant” E. coli exhibited differences in Km between 0.3 and 0.8 μM. The number of mutations compared to the laboratory E. coli was substantially lower compared to the other group.

Keywords

buthionine sulfoximine; Escherichia coli; glutathione; infections; swabs

Authors

KOMÍNKOVÁ, M.; KIZEK, R.; MICHÁLEK, P.; ČÍHALOVÁ, K.; GURÁŇ, R.; CERNEI, N.; NEJDL, L.; ŠMERKOVÁ, K.; DOSTÁLOVÁ, S.; CHUDOBOVA, D.; HEGER, Z.; VESELÝ, R.; GUMULEC, J.; KYNICKÝ, J.; XHAXHIU, K.; ZÍTKA, O.; ADAM, V.

RIV year

2015

Released

31. 5. 2015

Publisher

MDPI

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

16

Number

4

State

Swiss Confederation

Pages from

7210

Pages to

7229

Pages count

20

URL

http://www.mdpi.com/1422-0067/16/4/7210

Full text in the Digital Library

http://hdl.handle.net/11012/63706

BibTex

@article{BUT118729,
  author="Markéta {Komínková} and René {Kizek} and Petr {Michálek} and Kristýna {Číhalová} and Roman {Guráň} and Natalia Vladimirovna {Cernei} and Lukáš {Nejdl} and Kristýna {Šmerková} and Simona {Dostálová} and Dagmar {chudobova} and Zbyněk {Heger} and Radek {Veselý} and Jaromír {Gumulec} and Jindřich {Kynický} and Kledi {Xhaxhiu} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="Study of Linkage between Glutathione Pathway and the Antibiotic Resistance of Escherichia coli from Patients’ Swabs",
  journal="International Journal of Molecular Sciences",
  year="2015",
  volume="16",
  number="4",
  pages="7210--7229",
  doi="10.3390/ijms16047210",
  issn="1422-0067",
  url="http://www.mdpi.com/1422-0067/16/4/7210"
}