Publication detail

Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs

ČÍHALOVÁ, K. CHUDOBOVA, D. KIZEK, R. MICHÁLEK, P. MOULICK, A. GURÁŇ, R. KOPEL, P. ADAM, V.

Original Title

Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs

Type

journal article in Web of Science

Language

English

Original Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a dangerous pathogen resistant to β-lactam antibiotics. Due to its resistance, it is difficult to manage the infections caused by this strain. We examined this issue in terms of observation of the growth properties and ability to form biofilms in sensitive S. aureus and MRSA after the application of antibiotics (ATBs)—ampicillin, oxacillin and penicillin—and complexes of selenium nanoparticles (SeNPs) with these ATBs. The results suggest the strong inhibition effect of SeNPs in complexes with conventional ATBs. Using the impedance method, a higher disruption of biofilms was observed after the application of ATB complexes with SeNPs compared to the group exposed to ATBs without SeNPs. The biofilm formation was intensely inhibited (up to 99% ± 7% for S. aureus and up to 94% ± 4% for MRSA) after application of SeNPs in comparison with bacteria without antibacterial compounds whereas ATBs without SeNPs inhibited S. aureus up to 79% ± 5% and MRSA up to 16% ± 2% only. The obtained results provide a basis for the use of SeNPs as a tool for the treatment of bacterial infections, which can be complicated because of increasing resistance of bacteria to conventional ATB drugs.

Keywords

Staphylococcus aureus; methicillin-resistant Staphylococcus aureus; antibiotics; selenium nanoparticles

Authors

ČÍHALOVÁ, K.; CHUDOBOVA, D.; KIZEK, R.; MICHÁLEK, P.; MOULICK, A.; GURÁŇ, R.; KOPEL, P.; ADAM, V.

RIV year

2015

Released

16. 10. 2015

Publisher

MDPI

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

16

Number

10

State

Swiss Confederation

Pages from

24656

Pages to

24672

Pages count

17

URL

http://www.mdpi.com/1422-0067/16/10/24656

Full text in the Digital Library

http://hdl.handle.net/11012/62263

BibTex

@article{BUT118732,
  author="Kristýna {Číhalová} and Dagmar {chudobova} and René {Kizek} and Petr {Michálek} and Amitava {Moulick} and Roman {Guráň} and Pavel {Kopel} and Vojtěch {Adam}",
  title="Staphylococcus aureus and MRSA Growth and Biofilm Formation after Treatment with Antibiotics and SeNPs",
  journal="International Journal of Molecular Sciences",
  year="2015",
  volume="16",
  number="10",
  pages="24656--24672",
  doi="10.3390/ijms161024656",
  issn="1422-0067",
  url="http://www.mdpi.com/1422-0067/16/10/24656"
}