Publication detail

Flow Injection Electrochemical Analysis of Complexes of Influenza Proteins with CdS, PbS and CuS Quantum Dots

FIALOVÁ, D. KREJČOVÁ, L. JANŮ, L. BLAŽKOVÁ, I. KRYŠTOFOVÁ, O. HYNEK, D. KOPEL, P. DRBOHLAVOVÁ, J. KONEČNÁ, M. VACULOVIČOVÁ, M. KYNICKÝ, J. HUBÁLEK, J. BABULA, P. KIZEK, R. ADAM, V.

Original Title

Flow Injection Electrochemical Analysis of Complexes of Influenza Proteins with CdS, PbS and CuS Quantum Dots

Type

journal article in Web of Science

Language

English

Original Abstract

Due to threat coming from viral diseases, mainly from influenza, new assays and devices are investigated. This study is aimed at developing of low-cost isolation and detection of specific influenza protein hemagglutinin (HA) labelled with CdS, PbS, and CuS quantum dots. The influenza protein was determined via concentration of cadmium(II), lead(II), and copper(II) ions contained in quantum dots using stopped flow injection analysis with electrochemical detection (SFIA-ED). The main attention was devoted to detection of quantum dots and optimization of the whole analytical system. A particular aim was focused on an application of streptavidin-coated paramagnetic particles that were modified with biotinylated glycan. In addition, their selective binding properties to the influenza virus were monitored. Based on the results it can be concluded that CdS QDs were the best label for viral protein determination.

Keywords

Nanoparticles; Quantum Dots; Differential Pulse Voltammetry; Stopped Flow Injection Analysis; Glassy Carbon; Biosensors; Electrochemical Detection; Influenza Virus H5N1; Hemagglutinin

Authors

FIALOVÁ, D.; KREJČOVÁ, L.; JANŮ, L.; BLAŽKOVÁ, I.; KRYŠTOFOVÁ, O.; HYNEK, D.; KOPEL, P.; DRBOHLAVOVÁ, J.; KONEČNÁ, M.; VACULOVIČOVÁ, M.; KYNICKÝ, J.; HUBÁLEK, J.; BABULA, P.; KIZEK, R.; ADAM, V.

RIV year

2013

Released

1. 8. 2013

Publisher

ESG

Location

BORIVOJA STEVANOVICA 25-7, BELGRADE, 11000, SERBIA

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

8

Number

8

State

Republic of Serbia

Pages from

10805

Pages to

10817

Pages count

13

BibTex

@article{BUT120400,
  author="Dana {Fialová} and Ludmila {Krejčová} and Libor {Janů} and Iva {Blažková} and Olga {Kryštofová} and David {Hynek} and Pavel {Kopel} and Jana {Drbohlavová} and Marie {Konečná} and Markéta {Vaculovičová} and Jindřich {Kynický} and Jaromír {Hubálek} and Petr {Babula} and René {Kizek} and Vojtěch {Adam}",
  title="Flow Injection Electrochemical Analysis of Complexes of Influenza Proteins with CdS, PbS and CuS Quantum Dots",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2013",
  volume="8",
  number="8",
  pages="10805--10817",
  issn="1452-3981"
}