Publication detail

Novel biophysical determination of miRNAs related to prostate and head and neck cancers

HUDCOVÁ, K. TRNKOVÁ, L. KEJNOVSKÁ, I. VORLÍČKOVÁ, M. GUMULEC, J. KIZEK, R. MASAŘÍK, M.

Original Title

Novel biophysical determination of miRNAs related to prostate and head and neck cancers

Type

journal article in Web of Science

Language

English

Original Abstract

In this study we have chosen a new approach and characterized three miRNAs (miR-23a, miR-34a and miR-320a) related to prostate cancer and head and neck cancer by spectral (circular dichroic and UV-absorption spectra) and electrochemical (voltammetry at graphite and mercury electrodes) methods. The spectral and voltammetric results, reflecting different nucleotide sequences of miRNAs, were complemented by the results of DNAs(U) having the same oligonucleotide sequences as miRNAs. The effect of the substitution of ribose for deoxyribose was shown and structural diversity was confirmed. The stability of RNA and DNA(U) was studied using CD and UV-absorption spectroscopy and melting points were calculated. MiRNA-320a with the highest content of guanine provided the highest melting point. With respect to the rapid progress of miRNA electrochemical sensors, our results will be useful for the research and development of sensitive, portable and time-efficient miRNA sensors, which will be able to diagnose cancer and other diseases.

Keywords

miRNA · Circular dichroism · Electrochemistry · Prostate cancer · Head and neck cancer

Authors

HUDCOVÁ, K.; TRNKOVÁ, L.; KEJNOVSKÁ, I.; VORLÍČKOVÁ, M.; GUMULEC, J.; KIZEK, R.; MASAŘÍK, M.

RIV year

2015

Released

1. 4. 2015

ISBN

0175-7571

Periodical

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Year of study

44

Number

3

State

Federal Republic of Germany

Pages from

131

Pages to

138

Pages count

8

BibTex

@article{BUT120428,
  author="Kristýna {Hudcová} and Libuše {Trnková} and Iva {Kejnovská} and Michaela {Vorlíčková} and Jaromír {Gumulec} and René {Kizek} and Michal {Masařík}",
  title="Novel biophysical determination of miRNAs related to prostate and head and neck cancers",
  journal="EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS",
  year="2015",
  volume="44",
  number="3",
  pages="131--138",
  doi="10.1007/s00249-015-1008-y",
  issn="0175-7571"
}