Publication detail

Electrochemical Microarray as a Rapid Tool for Identification of Mutations in Influenza Virus Genes

MERLOS RODRIGO, M. KREJČOVÁ, L. HEGER, Z. ZÍTKA, O. ADAM, V. KIZEK, R.

Original Title

Electrochemical Microarray as a Rapid Tool for Identification of Mutations in Influenza Virus Genes

Type

journal article in Web of Science

Language

English

Original Abstract

Influenza viruses, as etiological agent of acute respiratory-related diseases, cause seasonal epidemics and/or less frequently pandemics with substantial mortality across the world. They display high mutation rates and complex evolutionary patterns inducing often significant resistance to antiviral drugs. We considered an electrochemical detection method, so called CombiMatrix ElectraSense core technology, to create one influenza array containing probes of all genes encodable by different subtypes of influenza type A genome. We detected particularly: neuraminidase, hemagglutinin, nucleocapsid protein, matrix protein 1, matrix protein 2, polymerase PB1, polymerase PA and nuclear export protein. Moreover, we succeeded to detect in vitro prepared mutations of the mentioned genes and it was found that the suggested array might be a suitable method not only for detection of the expression of the genes but also for detection of their mutations

Keywords

Influenza Virus; Microarray; Mutation Sequence; Antiviral Drug; Electrochemistry

Authors

MERLOS RODRIGO, M.; KREJČOVÁ, L.; HEGER, Z.; ZÍTKA, O.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

1. 12. 2015

Publisher

ESG

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

10

Number

12

State

Republic of Serbia

Pages from

9952

Pages to

9967

Pages count

16

URL

http://www.electrochemsci.org/papers/vol10/101209952.pdf

Full text in the Digital Library

http://hdl.handle.net/11012/201454

BibTex

@article{BUT120440,
  author="Miguel Ángel {Merlos Rodrigo} and Ludmila {Krejčová} and Zbyněk {Heger} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Microarray as a Rapid Tool for Identification of Mutations in Influenza Virus Genes",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2015",
  volume="10",
  number="12",
  pages="9952--9967",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/101209952.pdf"
}