Publication detail

Electrochemical Analysis of Metallothionein in Chicken Exposed to Cadmium Ions

TMEJOVÁ, K. HYNEK, D. KENŠOVÁ, R. BLAŽKOVÁ, I. VYSLOUŽILOVÁ, L. ŠTĚPÁNKOVÁ, O. POHANKA, M. ZEHNÁLEK, J. VACULOVIČOVÁ, M. ADAM, V. KIZEK, R.

Original Title

Electrochemical Analysis of Metallothionein in Chicken Exposed to Cadmium Ions

Type

journal article in Web of Science

Language

English

Original Abstract

Metallothionein as a protective protein has been studied in relationship to cancer diseases, toxicity of anticancer drugs and environmental pollution. Its electrochemical detection is based on Brdicka´s reaction suitable for the protein detection. The original reaction was invented by Rudolf Brdicka in 1930s and adapted protocol of the reaction was utilized in this study. We focused on the effect of cadmium ions contained in CdTe quantum dots in comparison to cadmium ions in a standard solution applied to chicken embryos. One day after application, chicken embryos were euthanized and liver, kidney, brain and muscle tissue were analyzed. The highest metallothionein levels were detected in the liver and kidney, which corresponded with cadmium accumulation determined by atomic absorption spectroscopy (68 μg/g liver tissue and 125 μg/g kidney tissue after application of quantum dots). The shape of electrochemical voltammograms was mathematically processed. In conclusion, metallothionein seems to be significantly involved in Cd detoxification mechanism.

Keywords

Brdicka´s reaction; Cd ions; Metallothionein; Electrochemistry; Toxicity

Authors

TMEJOVÁ, K.; HYNEK, D.; KENŠOVÁ, R.; BLAŽKOVÁ, I.; VYSLOUŽILOVÁ, L.; ŠTĚPÁNKOVÁ, O.; POHANKA, M.; ZEHNÁLEK, J.; VACULOVIČOVÁ, M.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

1. 5. 2015

Publisher

ESG

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

10

Number

5

State

Republic of Serbia

Pages from

3923

Pages to

3934

Pages count

12

URL

http://www.electrochemsci.org/papers/vol10/100503923.pdf

Full text in the Digital Library

http://hdl.handle.net/11012/201455

BibTex

@article{BUT121159,
  author="Kateřina {Tmejová} and David {Hynek} and Renáta {Kenšová} and Iva {Blažková} and Lenka {Vysloužilová} and Olga {Štěpánková} and Miroslav {Pohanka} and Josef {Zehnálek} and Markéta {Vaculovičová} and Vojtěch {Adam} and René {Kizek}",
  title="Electrochemical Analysis of Metallothionein in Chicken Exposed to Cadmium Ions",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2015",
  volume="10",
  number="5",
  pages="3923--3934",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/100503923.pdf"
}