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RAUDENSKÁ, M. SZTALMACHOVÁ, M. GUMULEC, J. FOJTŮ, M. POLANSKÁ, H. BALVAN, J. FEITH, M. BINKOVÁ, H. HORÁKOVÁ, Z. KOSTŘICA, R. KÍZEK, R. MASAŘÍK, M.
Original Title
Prognostic significance of the tumour-adjacent tissue in head and neck cancers
Type
journal article in Web of Science
Language
English
Original Abstract
Even with significant advances in operative skills and adjuvant therapies, the overall survival of patients suffering with head and neck squamous cancers (HNSCC) is unsatisfactory. Accordingly, no clinically useful prognostic biomarkers have been found yet for HNSCC. Many studies analysed the expression of potential markers in tumour tissues compared to adjacent tissues. Nevertheless, due to the sharing of the same microenvironment, adjacent tissues show molecular similarity to tumour tissues. Thus, gene expression patterns of 94 HNSCC tumorous tissues were compared with 31 adjacent tissues and with 10 tonsillectomy specimens of non-cancer individuals. The genes analysed at RNA level using quantitative RT-PCR and correlated with clinico-pathological conditions were as follows: EGF, EGFR, MKI67, BCL2, BAX, FOS, JUN, TP53, VEGF, FLT1, MMP2, MMP9, MT1A and MT2A. The elevated MT2A, BAX, EGF and JUN expression was associated with the influence of tumour cells on the rearrangement of healthy tissues, as well as a significant shift in the BAX/BCL2 ratio. Our investigation also indicated that adjacent tissues play an important role in cancerogenesis by releasing several tumour-supporting factors such as EGF. A gradual increase in the metallothionein expression, from the lowest one in tonsillectomy samples to the highest ones in tumour samples, suggests that MT expression might be tissue reaction to the presence of tumour cells. The results of this study confirmed the significance of metallothionein in tumori-genesis and gave evidences for its use as a potential HNSCC biomarker. Furthermore, this study highlighted the importance of histologically normal tumour-adjacent tissue in prediction of HNSCC progress.
Keywords
Head and neck neoplasms; Biological markers; Prognosis; Tumour microenvironment; Metallothionein; Matrix metalloproteinase 9; Gene expression
Authors
RAUDENSKÁ, M.; SZTALMACHOVÁ, M.; GUMULEC, J.; FOJTŮ, M.; POLANSKÁ, H.; BALVAN, J.; FEITH, M.; BINKOVÁ, H.; HORÁKOVÁ, Z.; KOSTŘICA, R.; KÍZEK, R.; MASAŘÍK, M.
RIV year
2015
Released
1. 11. 2015
ISBN
1010-4283
Periodical
TUMOR BIOLOGY
Year of study
36
Number
11
State
Swiss Confederation
Pages from
9929
Pages to
9939
Pages count
BibTex
@article{BUT123335, author="Martina {Raudenská} and Rom {Kostřica} and Markéta {Sztalmachová} and Jaromír {Gumulec} and Michaela {Fojtů} and Hana {Polanská} and Jan {Balvan} and Marek {Feith} and Hana {Binková} and Zuzana {Horáková} and René {Kizek} and Michal {Masařík}", title="Prognostic significance of the tumour-adjacent tissue in head and neck cancers", journal="TUMOR BIOLOGY", year="2015", volume="36", number="11", pages="9929--9939", doi="10.1007/s13277-015-3755-x", issn="1010-4283" }