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KŘÍŽKOVÁ, S. KEPINSKA, M. EMRI, G. MERLOS RODRIGO, M. TMEJOVÁ, K. NERUDOVÁ, D. KÍZEK, R. ADAM, V.
Original Title
Microarray analysis of metallothioneins in human diseases-A review
Type
journal article in Web of Science
Language
English
Original Abstract
Metallothioneins (MTs), low molecular mass cysteine-rich proteins, which are able to bind up to 20 monovalent and up to 7 divalent heavy metal ions are widely studied due to their functions in detoxification of metals, scavenging free radicals and cells protection against the oxidative stress. It was found that the loss of the protective effects of MT leads to an escalation of pathogenic processes and carcinogenesis. The most extensive area is MTs expression for oncological applications, where the information about gene patterns is helpful for the identification biological function, resistance to drugs and creating the correct chemotherapy. In other medical applications the effect of oxidative stress to cell lines exposed to heavy metals and hydrogen peroxide is studied as well as influence of drugs and cytokines on MTs expression and MTs expression in the adipose tissue. The precise detection of low metallothionein concentrations and its isoforms is necessary to understand the connection between quantity and isoforms of MTs to size, localization and type of cancer. This information is necessary for well-timed therapy and increase the chance to survival. Microarray chips appear as good possibility for finding all information about expression of MTs genes and isoforms not only in cancer, but also in other diseases, especially diabetes, obesity, cardiovascular diseases, ageing, osteoporosis, psychiatric disorders and as the effects of toxic drugs and pollutants, which is discussed in this review.
Keywords
Drug; Gene expression; Metallothionein; Microarray; Resistance; Tumour diseases
Authors
KŘÍŽKOVÁ, S.; KEPINSKA, M.; EMRI, G.; MERLOS RODRIGO, M.; TMEJOVÁ, K.; NERUDOVÁ, D.; KÍZEK, R.; ADAM, V.
Released
5. 1. 2016
ISBN
0731-7085
Periodical
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Year of study
117
Number
1
State
Kingdom of the Netherlands
Pages from
464
Pages to
473
Pages count
9
BibTex
@article{BUT123338, author="Soňa {Křížková} and Marta {Kepinska} and Gabriela {Emri} and Miguel Ángel {Merlos Rodrigo} and Kateřina {Tmejová} and Danuše {Nerudová} and René {Kizek} and Vojtěch {Adam}", title="Microarray analysis of metallothioneins in human diseases-A review", journal="JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS", year="2016", volume="117", number="1", pages="464--473", doi="10.1016/j.jpba.2015.09.031", issn="0731-7085" }