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HLAVATA, L. IGNAT, T. BLASKOVISOVA, J. RUTTKAY-NEDECKÝ, B. KOPEL, P. ADAM, V. KÍZEK, R. LABUDA, J.
Original Title
An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA
Type
journal article in Web of Science
Language
English
Original Abstract
A DNA-based biosensor is presented that can be applied to the detection of DNA damage caused by UV-C radiation (254 nm) in the presence of CdTe quantum dots (QDs). The sensor is composed of a glassy carbon electrode whose surface was modified with a layer of dsDNA and another layer of CdTe QDs. The response of this sensor is based on (a) the intrinsic anodic signal of the guanine moiety in the DNA that is measured by square-wave voltammetry, and (b) the cyclic voltammetric response of the redox indicator system hexacyanoferrate(III/II). Depending on the size of the QDs, they exert a significant effect on the rate of the degradation of dsDNA by UV-C light, and even by visible light. Time-dependent structural changes of DNA include opening of the double helix (as indicated by an increase in the redox response of the guanine moiety due to easy electron exchange with the electrode when compared to the original helix state and by an increase in the voltammetric peak current of the hexacyanoferrate(III/II) anion after degradation of the negatively charged DNA backbone on the electrode). The effects of QDs were verified for salmon sperm DNA and calf thymus DNA, and further corroborated by experiments in which DNA solutions were irradiated in the presence of QDs.
Keywords
DNA-based biosensor; Quantum dots; CdTe nanoparticles; UV-C radiation; DNA damage; Gel electrophoresis
Authors
HLAVATA, L.; IGNAT, T.; BLASKOVISOVA, J.; RUTTKAY-NEDECKÝ, B.; KOPEL, P.; ADAM, V.; KÍZEK, R.; LABUDA, J.
RIV year
2015
Released
1. 7. 2015
ISBN
0026-3672
Periodical
Microchimica Acta
Year of study
182
Number
9-10
State
Republic of Austria
Pages from
1715
Pages to
1722
Pages count
8
BibTex
@article{BUT123344, author="Lenka {Hlavata} and Teodora {Ignat} and Jana {Blaskovisova} and Branislav {Ruttkay-Nedecký} and Pavel {Kopel} and Vojtěch {Adam} and René {Kizek} and Jan {Labuda}", title="An electrochemical DNA-based biosensor to study the effects of CdTe quantum dots on UV-induced damage of DNA", journal="Microchimica Acta", year="2015", volume="182", number="9-10", pages="1715--1722", doi="10.1007/s00604-015-1502-z", issn="0026-3672" }