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KLUSOŇOVÁ, I. HORKÝ, P. SKLÁDANKA, J. KOMÍNKOVÁ, M. HYNEK, D. ZÍTKA, O. SKARPA, P. KIZEK, R. ADAM, V.
Original Title
An effect of various selenium forms and doses on antioxidant pathways at clover (Trifolium pratense L.)
Type
journal article in Web of Science
Language
English
Original Abstract
The aim of this study was to determine the effect of different doses and forms of foliar applied selenium on antioxidant indicators and selenium content in the forage of red clover (Trifolium pratense L.). This was done in the pot experiment, where the plants were placed in chambers under controlled-environment. In the experiment, different forms of selenium (sodium selenite, and/or sodium selenate), in concentrations corresponding to 2, 4 and 20 mg.m(-2) was used. Samples of aboveground tissues were taken at 14th, 28th and 42nd day after foliar application of selenium. The obtained samples were analysed using chromatographic and spectrophotometric techniques. After successful foliar application of selenium, the significant (P < 0.05) increase of the selenium content in aboveground mass of red clover was found. The maximum values for both forms were achieved in experimental groups treated with the highest concentration of selenium (20 mg.m(-2)). In comparison with selenate, selenite showed approximately 50% less efficient accumulation of selenium in the aboveground mass. From the determined antioxidants indicators, a positive effect in particular on the level of reduced glutathione was observed (P < 0.05) in both experimental groups (selenate, and/or selenite). The group treated with selenate had also higher content of phytochelatin 3 (P < 0.05). Despite the use of high selenium concentration (20 mg.m(-2)) its toxic effect on plants was not observed
Keywords
Selenium; Forage; Grass; Multi-Instrumental Analysis; Antioxidant
Authors
KLUSOŇOVÁ, I.; HORKÝ, P.; SKLÁDANKA, J.; KOMÍNKOVÁ, M.; HYNEK, D.; ZÍTKA, O.; SKARPA, P.; KIZEK, R.; ADAM, V.
RIV year
2015
Released
29. 12. 2015
Publisher
ESG
ISBN
1452-3981
Periodical
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Year of study
10
Number
12
State
Republic of Serbia
Pages from
9975
Pages to
9987
Pages count
13
URL
http://www.electrochemsci.org/papers/vol10/101209975.pdf
Full text in the Digital Library
http://hdl.handle.net/11012/201456
BibTex
@article{BUT123346, author="Iva {Klusoňová} and Pavel {Horký} and Jiří {Skládanka} and Markéta {Komínková} and David {Hynek} and Ondřej {Zítka} and Petr {Skarpa} and René {Kizek} and Vojtěch {Adam}", title="An effect of various selenium forms and doses on antioxidant pathways at clover (Trifolium pratense L.)", journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE", year="2015", volume="10", number="12", pages="9975--9987", issn="1452-3981", url="http://www.electrochemsci.org/papers/vol10/101209975.pdf" }