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HEGER, Z. MICHÁLEK, P. GURÁŇ, R. CERNEI, N. DUŠKOVÁ, K. VESELÝ, Š. ANÝZ, J. ŠTĚPÁNKOVÁ, O. ZÍTKA, O. ADAM, V. KIZEK, R.
Original Title
Differences in urinary proteins related to surgical margin status after radical prostatectomy
Type
journal article in Web of Science
Language
English
Original Abstract
Presented exploratory pilot study was aimed at evaluation of proteins present in urinary specimens collected from prostate cancer suffering subjects after radical prostatectomy, divided into two experimental cohorts: positive (n=15) and negative (n=15) surgical margins (PSM/NSM). The presence of PSM suggests inadequate cancer clearance and the possible need for additional treatment. Proper identification of these risk-patients is therefore of a paramount importance. Total protein profiles were firstly identified by using SDS-PAGE and compared by using partial least square discrimination analysis (PLS-DA), which revealed differences in molecular weights of 80-99 and 150-235 kDa between the experimental groups. For further identification of proteins, comparative proteomic technologies were employed. Two-dimensional gel electrophoresis with subsequent identification of protein spots by using MALDI-TOF mass fingerprinting revealed differential expression of proteins between NSM/PSM cohorts. Moreover, in PSM group, three uniquely identified proteins (cyclin-dependent kinase 6, galectin-3-binding protein and L-lactate dehydrogenase C chain) were found, which show tight connection with prostate cancer and presence of all of them was previously linked to certain aspects of prostate cancer. These proteins may be associated with the molecular mechanisms of prostate cancer development; hence, their identification may be helpful for the assessment of disease progression risk after radical prostatectomy, but also for possible early diagnosis.
Keywords
gel electrophoresis; mass spectrometry; proteomics; partial least squares discriminant analysis; prostate cancer
Authors
HEGER, Z.; MICHÁLEK, P.; GURÁŇ, R.; CERNEI, N.; DUŠKOVÁ, K.; VESELÝ, Š.; ANÝZ, J.; ŠTĚPÁNKOVÁ, O.; ZÍTKA, O.; ADAM, V.; KIZEK, R.
RIV year
2015
Released
9. 12. 2015
ISBN
1021-335X
Periodical
ONCOLOGY REPORTS
Year of study
34
Number
6
State
Hellenic Republic
Pages from
3247
Pages to
3255
Pages count
9
BibTex
@article{BUT123348, author="Zbyněk {Heger} and Petr {Michálek} and Roman {Guráň} and Natalia Vladimirovna {Cernei} and Kateřina {Dušková} and Štěpán {Veselý} and Jiří {Anýz} and Olga {Štěpánková} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kizek}", title="Differences in urinary proteins related to surgical margin status after radical prostatectomy", journal="ONCOLOGY REPORTS", year="2015", volume="34", number="6", pages="3247--3255", doi="10.3892/or.2015.4322", issn="1021-335X" }