Publication detail

Nanoparticles modified glassy carbon electrode coupled with microfluidic for nucleic acids analysis contained the repetitions of adenine

SKALIČKOVÁ, S. KOPEL, P. ZÍTKA, O. TRNKOVÁ, L. ADAM, V. KIZEK, R.

Original Title

Nanoparticles modified glassy carbon electrode coupled with microfluidic for nucleic acids analysis contained the repetitions of adenine

Type

journal article in Scopus

Language

English

Original Abstract

In this study we have used the microfluidic tool with a glassy carbon electrode modified with CuS nanoparticles for adenine determination using differential pulse voltammetry. The optimal parameters for adenine detection were the initial potential 0 V, and potential 1.8 V, step potential 0.015 V, amplitude 0.1 V, scan rate 0.07 V/s, pulse period 200 s and equilibration time 20 s. Prior the analysis the glassy carbon electrode (GCE) was modified by CuS nanoparticles. The optimal conditions for GCE modification were estimated as deposition potential –0.4 V for 200 s in the presence of 0.1M KCl solution containing 200 mM CuS nanoparticles. Subsequently the cyclic voltammetry was carried out in 0.1M NaOH solution repetitively for 20 cycles under potentials ranging from –0.5 to 0.3 V at 100 mV/s. The limit of detection (3 S/N) was estimated as 70 pg/cell volume. The method was applied for the analysis of oligonucleotides containing repetitions of adenine. We have found the dependence between number of adenine molecules in oligonucleotide and the electrochemical signal. This method might be useful for analysis of DNA sequences.

Keywords

adenin, elektrochemical detection, glassy carbon electrode, nanoparticles

Authors

SKALIČKOVÁ, S.; KOPEL, P.; ZÍTKA, O.; TRNKOVÁ, L.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

1. 12. 2015

ISBN

1213-7103

Periodical

Chemické listy

Year of study

109

Number

4

State

Czech Republic

Pages from

298

Pages to

304

Pages count

6

BibTex

@article{BUT123352,
  author="Sylvie {Skaličková} and Pavel {Kopel} and Ondřej {Zítka} and Libuše {Trnková} and Vojtěch {Adam} and René {Kizek}",
  title="Nanoparticles modified glassy carbon electrode coupled with microfluidic for nucleic acids analysis contained the repetitions of adenine",
  journal="Chemické listy",
  year="2015",
  volume="109",
  number="4",
  pages="298--304",
  issn="1213-7103"
}