Publication result detail

Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides

HEGER, Z.; MOULICK, A.; NGUYEN, H.; KREMPLOVÁ, M.; KOPEL, P.; HYNEK, D.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ZÍTKA, O.; ADAM, V.; KÍZEK, R.

Original Title

Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides

English Title

Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides

Type

WoS Article

Original Abstract

Carbon nanomaterials possess unique structural and physicochemical properties, and thus they are broadly utilized as carriers for a variety of therapeutic agents in nanomedicinal applications. Herein we present an electrochemical characterization of binding capability of acidic oxidized multi-walled carbon nanotubes (oMWCNTs) modified with poly(ethylene glycol) towards common cytostatic drug etoposide (or VP-16). The comparison of willingness of oMWCNTs and MWCNTs-PEG to form complexes with etoposide revealed significantly higher capacity in PEGylated variant of carbon nanotubes (the 15 mM etoposide loading capacity was approximately 46.4% in 2 mg of MWCNT-PEG or 28.1% in equal amount of oMWCNT). To obtain a multifunctional nanotransporter we employed the phosphorothioate oligodeoxynucleotide (PODN), which could further extend the possible biological effects of MWCNT-PEG-Etoposide complex. By using square wave voltammetry, the binding capacity of 2 mg of MWCNT-PEG-Etoposide (15 mM) towards PODNs was determined to be 4.5 mu M. Such characterized multifunctional complex can be further employed for biological testing on chemoresistant tumors, such as non-small-cell lung cancer, to enhance the treatment efficiency.

English abstract

Carbon nanomaterials possess unique structural and physicochemical properties, and thus they are broadly utilized as carriers for a variety of therapeutic agents in nanomedicinal applications. Herein we present an electrochemical characterization of binding capability of acidic oxidized multi-walled carbon nanotubes (oMWCNTs) modified with poly(ethylene glycol) towards common cytostatic drug etoposide (or VP-16). The comparison of willingness of oMWCNTs and MWCNTs-PEG to form complexes with etoposide revealed significantly higher capacity in PEGylated variant of carbon nanotubes (the 15 mM etoposide loading capacity was approximately 46.4% in 2 mg of MWCNT-PEG or 28.1% in equal amount of oMWCNT). To obtain a multifunctional nanotransporter we employed the phosphorothioate oligodeoxynucleotide (PODN), which could further extend the possible biological effects of MWCNT-PEG-Etoposide complex. By using square wave voltammetry, the binding capacity of 2 mg of MWCNT-PEG-Etoposide (15 mM) towards PODNs was determined to be 4.5 mu M. Such characterized multifunctional complex can be further employed for biological testing on chemoresistant tumors, such as non-small-cell lung cancer, to enhance the treatment efficiency.

Keywords

Antisense oligodeoxynucleotides; Carbon nanomaterials; Electrochemistry; Nanotechnology; Poly(ethylene) glycol

Key words in English

Antisense oligodeoxynucleotides; Carbon nanomaterials; Electrochemistry; Nanotechnology; Poly(ethylene) glycol

Authors

HEGER, Z.; MOULICK, A.; NGUYEN, H.; KREMPLOVÁ, M.; KOPEL, P.; HYNEK, D.; ECKSCHLAGER, T.; STIBOROVÁ, M.; ZÍTKA, O.; ADAM, V.; KÍZEK, R.

RIV year

2016

Released

01.09.2015

Publisher

ESG

ISBN

1452-3981

Periodical

International Journal of Electrochemical Science

Volume

10

Number

9

State

Republic of Serbia

Pages from

7707

Pages to

7719

Pages count

13

URL

http://www.electrochemsci.org/papers/vol10/100907707.pdf

Full text in the Digital Library

http://hdl.handle.net/11012/201457

BibTex

@article{BUT123363,
  author="Zbyněk {Heger} and Amitava {Moulick} and Hoai Viet {Nguyen} and Monika {Kremplová} and Pavel {Kopel} and David {Hynek} and Tomáš {Eckschlager} and Marie {Stiborová} and Ondřej {Zítka} and Vojtěch {Adam} and René {Kízek}",
  title="Characterization of multi-walled carbon nanotubes double-functionalization with cytostatic drug etoposide and phosphorothioate oligodeoxynucleotides",
  journal="International Journal of Electrochemical Science",
  year="2015",
  volume="10",
  number="9",
  pages="7707--7719",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/100907707.pdf"
}

Documents

100907707