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RUTTKAY-NEDECKÝ, B. SKALIČKOVÁ, S. KREMPLOVÁ, M. NEJDL, L. KUDR, J. HYNEK, D. NOVOTNÁ, M. LABUDA, J. ADAM, V. KIZEK, R.
Original Title
Formation of G-quadruplex and its utilizing for an automated spectrometric detection of cisplatin
Type
journal article in Web of Science
Language
English
Original Abstract
Here, we studied the formation of G-quadruplex using spectrophotometric methods (peroxidase activity of G-quadruplex) and verifications of obtained results by voltammetry (cytosine-adenine (CA) or guanine (G) peaks height). The results showed that the peroxidase activity of G-quadruplex increases with the increasing concentrations of K+ ions (1-20 mM) and Na+ ions (1-5 mM) with the highest values observed in the presence of K+ ions. The reverse trend was obtained with NH4+ ions, where the highest peroxidase activity was observed at the lowest concentration of NH4+ ions. The G-quadruplex formation was also proven electrochemically. The addition of 5 mM of K+ ions to the oligonucleotide solution with hemin caused a decrease of both determined peaks. The peroxidase activity of G-quadruplex increased with the increasing concentration of Ag+ ions within the range from 40 nM to 5 mu M. Further, the addition of different concentration of cisplatin (0.5-2 mu M) to the G-quadruplex was monitored. With the addition of cisplatin there was a decrease in peroxidase activity and simultaneously decrease in the CA peak height with the greatest effect in twentieth minute after the addition of cisplatin. The G-quadruplex coupling with gold magnetic nanoparticles was performed and the possibility of automation of spectrophotometric assay was proven
Keywords
G-quadruplex; Silver Ions; Cisplatin; Spectrophotometric Methods; Square Wave Voltammetry; Cyclic Voltammetry
Authors
RUTTKAY-NEDECKÝ, B.; SKALIČKOVÁ, S.; KREMPLOVÁ, M.; NEJDL, L.; KUDR, J.; HYNEK, D.; NOVOTNÁ, M.; LABUDA, J.; ADAM, V.; KIZEK, R.
RIV year
2015
Released
29. 5. 2015
Publisher
ESG
ISBN
1452-3981
Periodical
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Year of study
10
Number
5
State
Republic of Serbia
Pages from
3973
Pages to
3987
Pages count
14
URL
http://www.electrochemsci.org/papers/vol10/100503973.pdf
Full text in the Digital Library
http://hdl.handle.net/11012/201459
BibTex
@article{BUT123366, author="Branislav {Ruttkay-Nedecký} and Sylvie {Skaličková} and Monika {Kremplová} and Lukáš {Nejdl} and Jiří {Kudr} and David {Hynek} and Marie {Novotná} and Jan {Labuda} and Vojtěch {Adam} and René {Kizek}", title="Formation of G-quadruplex and its utilizing for an automated spectrometric detection of cisplatin", journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE", year="2015", volume="10", number="5", pages="3973--3987", issn="1452-3981", url="http://www.electrochemsci.org/papers/vol10/100503973.pdf" }