Publication detail

New generation Deep-UV LED for portable robust low cost optical detection in microfluidic and miniaturised analysis

DVOŘÁK, M.YAN, L.NUCHTAVORN, N.NESTERENKO, N. P.MACKA, M.

Original Title

New generation Deep-UV LED for portable robust low cost optical detection in microfluidic and miniaturised analysis

Type

abstract

Language

English

Original Abstract

Here we investigated a design philosophy of a portable medium-pressure LC system based on using off-the-shelf easily accessible components. The basis of the system is a modular microfluidic platform with the components assembled in a flexible manner on a breadboard (Fig.1). The components are syringe pumps and switch valves, joined with capillaries and microfluidic fittings, complemented with other components, including an injection valve and on-capillary detectors, all controlled by a PC. The pumping system is formed by miniaturised syringe pumps (5, 20 and 100µL options). Two pairs of pumps were used for each mobile phase to enable gradient elution capability, connected with two microfluidic switching valves, and then connected together through a Y-connector, thus providing a low hold-up volume for gradient formation. A 4 or 20 nL nano-LC sampling valve was used for the injection system, connecting to a capillary LC column (100 µm i.d., 50- 250 mm length, C18 RP monolith). Photometric detection was conducted through the capillary (100 µm i.d. PTFE-coated fused silica) with an LED-based UV-vis-NIR photometric detector, and/or an end-column electrochemical detector (amperometric and/or potentiometric). The characterisation of this portable LC system was conducted theoretically and experimentally, including the operating pressure, the performance in gradient mode and the performance of the detectors. The initial test analytes included primarily charged and uncharged food dyes of varying hydrophobicity.

Keywords

LED, miniaturisation, characterisation

Authors

DVOŘÁK, M.;YAN, L.;NUCHTAVORN, N.;NESTERENKO, N. P.;MACKA, M.

Released

31. 3. 2015

Publisher

Australia and New Zealand Nano and Microfluidic Symposium, Melbourne Centre for Nanofabrication

Location

Melbourne, Victoria, Australia

Pages from

18

Pages to

18

Pages count

19

URL

http://www.anznmf.org/

BibTex

@misc{BUT124061,
  author="DVOŘÁK, M. and YAN, L. and NUCHTAVORN, N. and NESTERENKO, N. P. and MACKA, M.",
  title="New generation Deep-UV LED for portable robust low cost optical detection in microfluidic and miniaturised analysis",
  booktitle="ANZNMF 2015 Program Schedule",
  year="2015",
  pages="18--18",
  publisher="Australia and New Zealand Nano and Microfluidic Symposium, Melbourne Centre for Nanofabrication",
  address="Melbourne, Victoria, Australia",
  url="http://www.anznmf.org/",
  note="abstract"
}