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HOLÍNKOVÁ, P. PEKAŘ, M.
Original Title
Fluorescence study of effect of hyaluronan addition to micelle solution of cetyltrimethylammonium bromide
Type
conference paper
Language
English
Original Abstract
This study deals with the effect of hyaluronan addition on the micellar formation of cationic surfactant cetyltrimethylammonium bromide (CTAB) in aqueous solution. Hyaluronan as negatively charged polyelectrolyte can interact with cationic micelles via electrostatic interactions to form aggregates, which could be potential used for targeted drug delivery. The effect of hyaluronan addition to CTAB solution was study by fluorescence measurement of two fluorescence probes located in different regions of micelles – hydrophobic perylene, which is solubilized in hydrophobic interior and hydrophilic one, fluorescein, which is located on the surface of cationic micelles due to its anionic character. The excitation and emission spectra, intensity of fluorescence, steady-state anisotropy of fluorescence and lifetime of probes in the samples were measured. It is observed, that the addition of hyaluronan to micellar solution of CTAB affected structural changes of microenvironment near the molecules of both fluorescence probes, which are reflected at increasing values of fluorescence anisotropy simultaneously with increasing amount of hyaluronan in solutions.
Keywords
fluorescence anisotropy, micelles, hyaluronan, cetyltrimethylammonium bromide, perylene, fluorescein
Authors
HOLÍNKOVÁ, P.; PEKAŘ, M.
RIV year
2015
Released
3. 12. 2015
Publisher
Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno
Location
Brno
ISBN
978-80-214-5290-9
Book
Studentská odborná konference Chemie je život 2015, Sborník příspěvků
Edition
první
Pages from
244
Pages to
250
Pages count
6
URL
http://www.fch.vut.cz/media/sok/sbornik_prispevku_2015.pdf
BibTex
@inproceedings{BUT126573, author="Petra {Holínková} and Miloslav {Pekař}", title="Fluorescence study of effect of hyaluronan addition to micelle solution of cetyltrimethylammonium bromide", booktitle="Studentská odborná konference Chemie je život 2015, Sborník příspěvků", year="2015", series="první", pages="244--250", publisher="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno", address="Brno", isbn="978-80-214-5290-9", url="http://www.fch.vut.cz/media/sok/sbornik_prispevku_2015.pdf" }