Publication detail

Separation and detection of bioconjugated quantum dots using on a chip electrophoresis

PEJOVIC SIMEUNOVIC, J. HUBÁLEK, J.

Original Title

Separation and detection of bioconjugated quantum dots using on a chip electrophoresis

Type

conference paper

Language

English

Original Abstract

Semiconductor nanocrystals, quantum dots (QDs), are nanoscale particles that have been attract a lot of attention due to their unique optic and electronic properties. Due to very diverse and numerous applications, it is really important to make a tool for precise and controlled detection of QDs. In this study, we investigate on a chip detection and separation of QDs bioconjugated with BSA (bovine serum albumin) using homemade equipment based on principle of capillary electrophoresis (CE) and optical detection. Quenching effect of different concentration of BSA on fluorescence intensity of QDs was monitored. It was found that with increasing concentration of BSA fluorescence intensity of QDs is decreasing. This research can lead to a better understanding of interaction between different size QDs and biomolecules.

Keywords

Quantum dots, bovine serum albumin, capillary electrophoresis, bioconjugation

Authors

PEJOVIC SIMEUNOVIC, J.; HUBÁLEK, J.

Released

28. 4. 2016

Publisher

Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních

Location

Brno

ISBN

978-80-214-5350-0

Book

Proceedings of the 22nd Conference STUDENT EEICT 2016

Edition

první

Pages from

660

Pages to

664

Pages count

5

URL

http://www.feec.vutbr.cz/EEICT/2016/sbornik/EEICT-2016-sborn%C3%ADk-komplet.pdf

BibTex

@inproceedings{BUT126581,
  author="Jelena {Pejović Simeunović} and Jaromír {Hubálek}",
  title="Separation and detection of bioconjugated quantum dots using on a chip electrophoresis",
  booktitle="Proceedings of the 22nd Conference STUDENT EEICT 2016",
  year="2016",
  series="první",
  pages="660--664",
  publisher="Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních",
  address="Brno",
  isbn="978-80-214-5350-0",
  url="http://www.feec.vutbr.cz/EEICT/2016/sbornik/EEICT-2016-sborn%C3%ADk-komplet.pdf"
}