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PEJOVIC SIMEUNOVIC, J. PEKÁRKOVÁ, J. HUBÁLEK, J.
Original Title
Separation and detection of bioconjugated QDs in microfluidic devices using capillary electrophoresis
Type
abstract
Language
English
Original Abstract
Semiconductor nanocrystals, also known as quantum dots (QDs), are nanoscale inorganic particles, and have been attract a lot of attention due to their unique optic and electronic properties. One of the most important characteristic is their bioconjugation with different biological materials such as protein, peptides and nucleic acid. Due to very diverse and numerous applications, it is really important to make a tool for precise and controlled detection of QDs. In this study, we investigate on-a-chip detection and separation of QDs. Separation is done by capillary electrophoresis using high voltage and as a carrier fluid, different buffers. Detection is done using UV filter and photodetector including high voltage photomultiplier. In first step mold, microchannels width of 50 µm was made using silicon wafer and photolithography. Next, mixture of the PDMS was completely degassed and pours onto the wafer mold to create transparent, flexible chip. Our first results showed that this method can be used to detected QDs, and separate QDs of different size as well as separate QDs bioconjugated with different biological material. Capillary electrophoresis is powerful separation technique that has been very useful in the analysis of biological material. What characterize this method are simplicity, short analysis time, small sample and reagent requirements, high separation efficient.
Keywords
QDs, microfluidics, bioconjugation
Authors
PEJOVIC SIMEUNOVIC, J.; PEKÁRKOVÁ, J.; HUBÁLEK, J.
Released
23. 4. 2015
Publisher
Masarykova univerzita
Location
Brno
ISBN
978-80-210-7825-3
Book
CEITEC PhD Retreat
Edition
1
Edition number
Pages from
109
Pages to
Pages count
BibTex
@misc{BUT126582, author="Jelena {Pejović Simeunović} and Jana {Pekárková} and Jaromír {Hubálek}", title="Separation and detection of bioconjugated QDs in microfluidic devices using capillary electrophoresis", booktitle="CEITEC PhD Retreat", year="2015", series="1", edition="1", pages="109--109", publisher="Masarykova univerzita", address="Brno", isbn="978-80-210-7825-3", note="abstract" }