Publication detail

Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments

GALANDÁKOVÁ, A. ULRICHOVÁ, J. LANGOVÁ, K. HANÁKOVÁ, A. VRBKA, M. HARTL, M. GALLO, J.

Original Title

Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments

Type

journal article in Web of Science

Language

English

Original Abstract

Wear testing of total joint replacement (TJR) is mandatory in preclinical testing before implantation of TJR into the human body. Testing is governed by current international standards that recommend bovine serum (BS) as a lubricating fluid to replace synovial fluid (SF). Recently, the use of BS has been criticized because of differences in content, fluid characteristics, and nonhuman origin. As a result, a more realistic lubricant mimicking SF is needed. To define SF composition, we analyzed SF obtained during revisions of total hip and knee arthroplasties and compared it with SF obtained during primary arthroplasties and from patients without TJR. Samples were acquired from 152 patients. We found that the median total protein concentration for all SF was 36.8 mg/mL, which is significantly higher than concentrations currently recommended by the ISO standards. The gama globulin concentration was significantly higher and the phospholipid concentration significantly lower in patients with revision of TJR compared with patients without TJR. No significant difference was found in hyaluronic acid concentration and viscosity among the groups. Our results support the need to improve the definition of a more clinically relevant wear testing lubricant in the ISO standards.

Keywords

total hip and knee replacement, protein, hyaluronic acid, phospholipid, viscosity

Authors

GALANDÁKOVÁ, A.; ULRICHOVÁ, J.; LANGOVÁ, K.; HANÁKOVÁ, A.; VRBKA, M.; HARTL, M.; GALLO, J.

Released

18. 4. 2016

Publisher

John Wiley & Sons Inc.

Location

USA

ISBN

1552-4981

Periodical

Journal of biomedical materials research. Part B, Applied biomaterials (Electronic)

Year of study

105

Number

6

State

United States of America

Pages from

1422

Pages to

1431

Pages count

10

URL

http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33663/full

BibTex

@article{BUT126663,
  author="GALANDÁKOVÁ, A. and ULRICHOVÁ, J. and LANGOVÁ, K. and HANÁKOVÁ, A. and VRBKA, M. and HARTL, M. and GALLO, J.",
  title="Characteristics of synovial fluid required for optimization of lubrication fluid for biotribological experiments",
  journal="Journal of biomedical materials research. Part B, Applied biomaterials (Electronic)",
  year="2016",
  volume="105",
  number="6",
  pages="1422--1431",
  doi="10.1002/jbm.b.33663",
  issn="1552-4981",
  url="http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33663/full"
}