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OBRUČA, S. SEDLÁČEK, P. KRZYZANEK, V. MRAVEC, F. HRUBANOVA, K. SAMEK, O. KUČERA, D. BENEŠOVÁ, P. MÁROVÁ, I.
Original Title
Accumulation of Poly(3-hydroxybutyrate) Helps Bacterial Cells to Survive Freezing
Type
journal article in Web of Science
Language
English
Original Abstract
Accumulation of polyhydroxybutyrate (PHB) seems to be a common metabolic strategy adopted by many bacteria to cope with cold environments. This work aimed at evaluating and understanding the cryoprotective effect of PHB. At first a monomer of PHB, 3-hydroxybutyrate, was identified as a potent cryoprotectant capable of protecting model enzyme (lipase), yeast (Saccharomyces cerevisiae) and bacterial cells (Cupriavidus necator) against the adverse effects of freezing-thawing cycles. Further, the viability of the frozen-thawed PHB accumulating strain of C. necator was compared to that of the PHB non-accumulating mutant. The presence of PHB granules in cells was revealed to be a significant advantage during freezing. This might be attributed to the higher intracellular level of 3-hydroxybutyrate in PHB accumulating cells (due to the action of parallel PHB synthesis and degradation, the so-called PHB cycle), but the cryoprotective effect of PHB granules seems to be more complex. Since intracellular PHB granules retain highly flexible properties even at extremely low temperatures (observed by cryo-SEM), it can be expected that PHB granules protect cells against injury from extracellular ice. Finally, thermal analysis indicates that PHB-containing cells exhibit a higher rate of transmembrane water transport, which protects cells against the formation of intracellular ice which usually has fatal consequences.
Keywords
Polyhydroxybutyrate, 3-hydroxybuyrate, cryoprotectants, intracellular water, stress response
Authors
OBRUČA, S.; SEDLÁČEK, P.; KRZYZANEK, V.; MRAVEC, F.; HRUBANOVA, K.; SAMEK, O.; KUČERA, D.; BENEŠOVÁ, P.; MÁROVÁ, I.
Released
17. 6. 2016
Publisher
PLoS
ISBN
1932-6203
Periodical
PLOS ONE
Year of study
11
Number
6
State
United States of America
Pages from
1
Pages to
16
Pages count
URL
http://europepmc.org/articles/PMC4912086?pdf=render
Full text in the Digital Library
http://hdl.handle.net/11012/65334
BibTex
@article{BUT126803, author="OBRUČA, S. and SEDLÁČEK, P. and KRZYZANEK, V. and MRAVEC, F. and HRUBANOVA, K. and SAMEK, O. and KUČERA, D. and BENEŠOVÁ, P. and MÁROVÁ, I.", title="Accumulation of Poly(3-hydroxybutyrate) Helps Bacterial Cells to Survive Freezing", journal="PLOS ONE", year="2016", volume="11", number="6", pages="1--16", doi="10.1371/journal.pone.0157778", issn="1932-6203", url="http://europepmc.org/articles/PMC4912086?pdf=render" }